バイオインスピレーションによるナノ酵素ベースのH2O2ホメオスタシス破壊器の構築
Yanjuan Sang1,2, Fangfang Cao1,2, Wei Li1,3
1Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.
Journal of the American Chemical Society
|February 27, 2020
まとめ
この研究は,癌細胞における過酸化水素 (H2O2) ホメオスタシスを破壊するナノ酵素を開発した. H2O2の生成を増加させ,排出を減少させることで,化学動力療法 (CDT) の有効性を高めます.
科学分野:
- バイオマテリアル科学
- ナノテクノロジー
- ガン 治療
背景:
- 細胞内過酸化水素 (H2O2) の不足は,腫瘍における化学動力療法 (CDT) の有効性を制限する.
- CDTのためのH2O2を確実に供給する薬剤の開発は依然として大きな課題です.
研究 の 目的:
- ナノ酵素ベースの H2O2 ホメオスタシス破壊剤を設計し,細胞内H2O2レベルを上昇させ,CDTを強化する.
- 腫瘍細胞のH2O2バランスを操作することで 癌の治療を改善する 新しい戦略を研究する
主な方法:
- スーパーオキシードジスミュータゼを模倣する活性を持つナノ酵素を製造し,スーパーオキシードラジカル (O2•−) をH2O2に変換する.
- H2O2の分解を抑制するために,カタラゼの活性抑制とグルタチオンの枯渇.
- H2O2 ホメオスタシスを妨害し,がん細胞におけるフェントン様反応を強化するナノ酵素の能力の評価.
主要な成果:
- ナノ酵素はO2•−を変換することで細胞内H2O2の産生を成功裏に増加させた.
- 破壊物質はH2O2の排出経路を効果的に阻害し,H2O2の蓄積につながった.
- 高いH2O2レベルはCDTの効率を大幅に高めました
結論:
- 開発されたナノ酵素はH2O2ホメオスタシス破壊体として作用し,CDTを改善するために細胞内H2O2を効果的に増加させます.
- この研究は,先進的なCDT剤を設計するための有望なアプローチを提示し,がん治療のためのバイオインスピレーション材料設計の洞察を提供します.
関連する概念動画
01:29Methods of Sterilization I: Physical Methods
25.2K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
25.2K
01:30Methods of Sterilization II: Chemical Methods
9.5K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
9.5K
01:32Tissue Homogenization and Cell Lysis
9.0K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
9.0K
01:23Physical Methods for Controlling Microbial Growth: Temperature
1.7K
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
1.7K
01:27Chemical Agents for Microbial Control
1.6K
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
1.6K
01:28Microbial Bioremediation of Pesticides
85
Pesticides often feature structurally complex chemical architectures, incorporating halogen groups and multiple aromatic rings. These characteristics confer high chemical stability, rendering many pesticides resistant to natural degradation processes. This resistance poses significant environmental concerns, as persistent pesticide residues can accumulate in ecosystems and affect non-target organisms.Despite the inherent stability of many pesticides, certain microorganisms possess the metabolic...
85