ヒトACE2の全身長によるSARS-CoV-2の認識のための構造的基礎
Renhong Yan1,2, Yuanyuan Zhang1,2, Yaning Li3
1Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China.
Science (New York, N.Y.)
|March 6, 2020
まとめ
重症急性呼吸器症候群コロナウイルス (SARS-CoV) とSARS-CoV-2は,血管新生変換酵素2 (ACE2) を細胞受容体として使用する. クリオ電子顕微鏡では,SARS-CoV-2に結合するACE2の構造が明らかになり,ウイルスの侵入のための分子相互作用が詳細に示されました.
科学分野:
- 構造生物学
- ウイルス学
- 感染症の分子メカニズム
背景:
- アンジオテンシン変換酵素2 (ACE2) は重要な細胞受容体である.
- ACE2は,COVID-19の原因となるウイルスであるSARS-CoVとSARS-CoV-2の侵入を容易にする.
研究 の 目的:
- 人間のACE2の冷凍電子顕微鏡構造を決定する.
- ACE2とSARS-CoV-2のスパイクタンパク質受容体結合領域 (RBD) の相互作用を解明する.
主な方法:
- 高解像度構造を取得するために,冷凍電子顕微鏡 (冷凍EM) が使用されました.
- 構造は,SARS-CoV-2 RBDを含むおよび含まれないACE2-B0AT1複合体のために決定された.
主要な成果:
- ACE2-B0AT1複合体はヘテロディメールの二重体を形成する.
- 高解像度構造 (全体で2. 9 Å,インターフェースで3. 5 Å) は,ACE2とSARS-CoV-2RBDの結合インターフェースを明らかにします.
- ACE2は主に細胞外ペプチダース領域の極性残基を通してRBDを認識する.
結論:
- この研究は,SARS-CoV-2がACE2に結合する方法に関する詳細な分子洞察を提供します.
- これらの相互作用を理解することは,新型コロナウイルス感染症に対する戦略の開発に不可欠です.
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