哺乳類の脳からのV-ATPaseの構造

Yazan M Abbas¹, Di Wu², Stephanie A Bueler¹

¹Molecular Medicine Program, The Hospital for Sick Children Research Institute, Toronto, ON M5G 0A4, Canada.

Science (New York, N.Y.)

|March 14, 2020

まとめ

研究者らはネズミの脳のV-ATPaseの構造を明らかにしましたこれは神経伝達物質の充電に不可欠な酵素です酵素を検出しました

科学分野:

神経科学
生物化学
構造生物学

背景:

膀型または真空型のアデノシントリフォスファタゼ (V-ATPases) は,神経細胞のシナプス膀に神経伝達物質の負荷を活性化します.
V-ATPase複合体は多数のサブユニットイソフォームを有し,構造的および機能的分析に課題をもたらす.

研究の目的:

ネズミの脳V-ATPASEの原子構造を決定する
V-ATPaseの組立と機能のメカニズムを解明する.

主な方法:

レギオネラ・プネモフィラ効果タンパク質SidKを用いた均質のラット脳V-ATPaseの分離
原子モデルの構築のための冷凍電子顕微鏡 (冷凍EM).

主要な成果:

V-ATPaseの原子模型が構築され,ATP:陽子の比率は3:10であった.
酵母子ユニットfのホモログは,暫定的にRNAseKと特定され,膜領域で発見された.
cリングアンカーはATP6AP1/Ac45とATP6AP2/PRRを分割し,後者は (プロレニン受容体である.

結論:

決定された構造は,ATP6AP1/Ac45とATP6AP2/PRRによるV-ATPase触媒および膜領域の組み立てに関する洞察を提供します.
この発見は,V-ATPaseの機能と調節における関連タンパク質の役割を明確にします.

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