非レドックス活性タンパク質における電子導電共鳴

Bintian Zhang¹, Weisi Song¹, Jesse Brown²

¹Biodesign Institute, Arizona State University, Tempe, Arizona 87287, United States.

Journal of the American Chemical Society

|March 17, 2020

まとめ

研究者は非リドックス活性タンパク質を研究し電気の伝導性を発見しました導電共鳴が観察され,これらの生物学的分子における長距離電荷輸送のための新しいメカニズムが示唆されました.

科学分野:

バイオ電子
分子生物物理学
タンパク質伝導

背景:

バイオエレクトロニクスの研究は伝統的に生物学的電荷輸送のための酸化還元活性タンパク質に焦点を当てています.
レドックスタンパク質の電子伝導性は,その特定のレドックスポテンシャルでピークに達します.
最近の発見は,非レドックス活性タンパク質も有意な電子伝導性を示すことを示しているが,そのメカニズムは不明である.

研究の目的:

非リドックス活性タンパク質の電子伝導のメカニズムを調査する.
制御された電子注入下で非リドックス活性タンパク質の単分子伝導性を測定する.
酸化還元活性がないタンパク質における電荷輸送を制御する要因を理解する.

主な方法:

単一分子伝導度測定
溶液中のタンパク質の潜在静止制御
探査器の伝導性特性を変化させる電子注入エネルギー

主要な成果:

3つの非レドックス活性タンパク質を測定した.
すべてのテストされたタンパク質に対して一貫した導電共鳴が観察されました.
この共鳴は,最も近いアミノ酸酸化ポテンシャルから約0. 7Vの電子注入ポテンシャルで発生しました.

結論:

観察された伝導性共鳴は,非レドックス活性タンパク質における新しい電荷輸送メカニズムを示唆する.
潜在的シフトは,タンパク質内部の再構成エネルギーが減少していることを示します.
この発見は,内部に電荷媒体が注入されたときのタンパク質の長距離伝導性を説明できる.

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