セントロソームの固定は,先駆体特性と皮質形成を調節する
Wei Shao1,2, Jiajun Yang3, Ming He3,4
1Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Centre, New York, NY, USA.
Nature
|April 3, 2020
まとめ
センターソームを頂上膜に固定することで,放射性膠原細胞 (RGP) の機械的性質が制御される. これはRGPの増殖を制御し,哺乳類の脳皮質のサイズと折り畳みに影響を与えます.
科学分野:
- 神経科学
- 発達生物学
- 細胞生物学
背景:
- 放射性膠原細胞 (RGP) は,哺乳類の発達中の脳皮質におけるニューロンと膠原細胞の生成に不可欠である.
- RGPにおけるセンターソームの頂点位置は,未知の分子基盤と機能を持つユニークなサブセルラー組織である.
研究 の 目的:
- 皮質のRGPにおけるアピカル膜へのセンターソームアンカリングの分子メカニズムと機能的意義を調査する.
- RGPの行動と脳皮質の発達におけるセンターソーム組織の役割を明らかにする.
主な方法:
- 中心体タンパク質83 (CEP83) の機能を研究するためにマウスモデルを使用した.
- センターソームの固定,細胞力学,RGPの増殖に対するCEP83除去の影響を調査した.
- 変化したセンターソーム組織への反応として,YAP信号経路の関与を評価した.
主要な成果:
- CEP83は,RGPの頂上膜に母中心球を固定する遠端付属体の形成に不可欠です.
- CEP83の除去はセンターソームの固定を乱し,マイクロチューブルの組織化とアピカル膜のメカニズムを変化させます.
- CEP83の枯渇はYAPのシグナル伝達を活性化し,過剰なRGPの増殖,中間原始細胞の増加,および拡大,異常な折りたたみした皮質を引き起こします.
- YAPの同時除去は,CEP83の除去によって引き起こされた皮質の拡大と折り畳み欠陥を救済します.
結論:
- 皮質のRGPの機械的性質を調節するために,アピカル膜へのセンターソームの固定が重要です.
- この機械的調節はRGPのミトーシス行動,原始細胞の産生,そして最終的には哺乳類の脳皮質の大きさと複雑な折り畳みに影響を与えます
- 神経元細胞の行動と脳の発達を制御するメカニカル伝達経路におけるセントロソームの新たな役割を明らかにする.
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