関連する実験動画

Updated: May 6, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

シーフベース水解の背後には何が隠されていますかシアライテッド・グリカンの正確な捕獲のためのダイナミック・コバルント・ケミストリー

Yuting Xiong^1,2, Xiuling Li¹, Minmin Li^1,2

¹CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, P. R. China.

Journal of the American Chemical Society

|April 4, 2020

まとめ

新しいシフ塩基水解法では,がんバイオマーカーの発見に不可欠なシアライテッドグリカン (SGs) を正確に捕獲します. このダイナミックな共性化学のアプローチは,グリコプロテオミクスとシアル酸の検出を強化します.

科学分野:

生物化学
分析化学
材料科学

背景:

シアライテッドグリカン (SGs) の異常表現は,がんの進行と転移に関連しています.
シアリ化グリコプロテインは,がんのバイオマーカーとして確立されています.
SGの識別と分析の現在の方法は複雑で,分析前のサンプル準備の改善が必要である.

研究の目的:

質量スペクトロメトリー (MS) 分析の前に生物サンプルからSGを捕獲するための革新的で効果的な方法を開発する.
精密なSG濃縮のためにダイナミック・コバルント化学を活用する.

主な方法:

新しいシフ塩基水解戦略が採用された.
グルコピラノシド-シフ塩基改変シリカゲルが合成され,特徴づけられました.
シアライテッドのグリコペプチドを捕獲する材料の性能は,選択性,容量,および回復のために評価されました.

主要な成果:

開発された材料は,異常な濃縮選択性 (干渉による1:5000比) を示した.
シアライスされたグリコペプチドの高吸収能力 (120 mg·g−1) と濃縮回復 (95. 5%) が達成された.
シーフ基材は水解が容易で,濃縮材料ではユニークな特徴を示した.

結論:

シーフ塩基水解戦略は,高精度,効率,軽度,およびSG捕獲のための可逆的なアプローチを提供します.
この方法は,グリコプロテオミクスとシアル酸の検出を大幅に進める.
このアプローチは癌のバイオマーカーの発見にかなりの期待を寄せています

さらに関連する動画

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines

Published on: November 25, 2017

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

関連する概念動画

Hydrolysis

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...

Chemistry of Carbohydrates

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Preparation and Reactions of Thiols

Preparation and Reactions of Thiols

Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.

Aldehydes and Ketones with Water: Hydrate Formation

Aldehydes and Ketones with Water: Hydrate Formation

An oxygen-based nucleophile, like water, can undergo addition reactions with aldehydes and ketones. The reaction leads to the formation of hydrates, also referred to as 1,1-diols or geminal diols.
The formation of hydrates is a reversible reaction. Hydrate formation is influenced by steric and electronic factors accompanying the alkyl substituents on the carbonyl group: The rate of hydrate formation increases with a decrease in the number of alkyl groups attached to the carbonyl carbon. Hence,...

Acid Halides to Carboxylic Acids: Hydrolysis

Acid Halides to Carboxylic Acids: Hydrolysis

Hydrolysis of acid halides is a nucleophilic acyl substitution reaction in which acid halides react with water to give carboxylic acids. The reaction occurs readily and does not require acid or a base catalyst.
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields...