膀媒介による非従来のタンパク質分泌のための転位経路
Min Zhang1, Lei Liu1, Xubo Lin2
1State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Cell
|April 10, 2020
まとめ
研究者らは,TMED10がタンパク質チャネルとして働き,膀輸送経由でリードレスタンパク質の分泌を促進することを発見しました. この経路は,非従来的なタンパク質の分泌に不可欠な,ベシクルへの貨物の入り口を調節します.
科学分野:
- 細胞生物学
- 分子生物学
- タンパク質の分泌
背景:
- リードレスタンパク質は,通常は膀を含む,従来とは異なる経路で分泌されます.
- リーダーのない貨物のベシクルへの入り口のメカニズムは 十分に理解されていない.
- 膀の流通は細胞分泌の重要なプロセスです
研究 の 目的:
- 導体なしの荷物が分泌のために膀に入るメカニズムを解明する.
- リードレス・カーゴ・ベシクルの侵入を 制御する分子要素を特定する
- 非従来のタンパク質分泌におけるタンパク質チャネルの役割を理解する.
主な方法:
- リポソームを用いた in vitro 溶解測定法
- TMED10の細胞局所化研究
- タンパク質と荷物の相互作用とオリゴメリゼーションの分析
- 貨物の移動における HSP90の役割を調査しています
主要な成果:
- TMED10は,リードレス・カーゴ・ベシクル・エントリーを媒介するタンパク質・チャネルとして特定された.
- TMED10 C端領域は,選択的な放出のための貨物モチーフと相互作用します.
- TMED10は leaderless cargoを in vitroで直接リポソームに変換する.
- トランスロケーションはタンパク質展開に依存し,HSP90が強化されます.
- TMED10は ER-Golgiの中間のコンパートメントに位置し,貨物の入りを指示します.
- カーゴ結合はTMED10のホモオリゴメリゼーションを誘導し,潜在的に転位チャネルを形成する.
結論:
- TMED10は,リードレス貨物分泌の転位経路の重要な構成要素です.
- TMED10-cargoの相互作用により,選択的にタンパク質が放出されます.
- TMED10は,おそらくホモオリゴーマーとして,膀への入り口として機能する.
- この発見は 非伝統的なタンパク質分泌の 根本的なメカニズムに光を当てました
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