G3BP1は,ストレスの粒子を組み立てるために相分離をトリガーする調整可能なスイッチです

Peiguo Yang¹, Cécile Mathieu², Regina-Maria Kolaitis¹

¹Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.

Cell

|April 18, 2020

まとめ

ストレス粒子は,G3BP1タンパク質によって駆動される液体液相分離 (LLPS) により組み立てられる. 乱れた領域とリン酸化が,他の結合因子によって調節されるこのプロセスを制御します.

科学分野:

細胞生物学
生物化学
バイオ物理学

背景:

リボヌクレオプロテイン (RNP) 粒子の組成,特にストレス粒子の (SGs) 仕組みは不明である.
SGは,ストレスに反応する真核細胞のダイナミックで可逆的な細胞質集合体である.

研究の目的:

SGアセンブリのメカニズムを解明する.
SGの形成と構成の主要な規制者を特定する.

主な方法:

液体液相分離 (LLPS) がSG組立のためのメカニズムとして調査されました.
SG形成における中心タンパク質としてのG3BP1の役割を特徴づけた.
G3BP1の調節における本質的に乱れた領域 (IDR) とリン酸化の機能を分析した.

主要な成果:

SGは,コアタンパク質-RNA相互作用ネットワークによって駆動されるLLPSを通じて組み立てられます.
G3BP1は分子スイッチとして働き,RNA依存のLLPSを誘発する.
G3BP1のLLPS傾向は,そのIDR,リン酸化,および外部結合因子によって調節されます.

結論:

SGアセンブリはLLPSによって制御され,G3BP1が重要なレギュレータである.
G3BP1の構造的な特徴と提携関係が SGの形成を調整する.
この研究は,RNPの粒子のダイナミクスの生体物理的原理の洞察を提供します.

キーワード:

G3BP1 について本質的に無秩序なタンパク質生物分子凝縮物協同活動コア・ストレス・グラニュル・ネットワーク液体-液体相分離膜のない器官分子スイッチマルチバレンシーストレス・グラニュル

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