SARS-CoV-2 タンパク質の相互作用マップは,薬物の再利用の標的を明らかにしています
David E Gordon1,2,3,4, Gwendolyn M Jang1,2,3,4, Mehdi Bouhaddou1,2,3,4
1QBI COVID-19 Research Group (QCRG), San Francisco, CA, USA.
Nature
|May 1, 2020
まとめ
研究者らは,SARS-CoV-2とヒトの細胞間の332のタンパク質相互作用を特定した. COVID-19に対する抗ウイルス活性を示す2種類の薬剤で,薬効性のあるヒトタンパク質を標的とした69の化合物を発見した.
科学分野:
- ウイルス学
- 分子生物学
- 薬物の発見
背景:
- 深刻な急性呼吸器症候群の新型コロナウイルス2型 (SARS-CoV-2型) は,承認された治療法やワクチンのない世界的なパンデミックであるCOVID-19を引き起こす.
- SARS-CoV-2の細胞感染メカニズムを理解することは,効果的な治療法の開発に不可欠です.
研究 の 目的:
- SARS-CoV-2とヒトのタンパク質との宿主-病原体相互作用を特定する.
- COVID-19 治療のための潜在的な薬標的と抗ウイルス化合物を発見する.
主な方法:
- 人間の細胞で26のSARS-CoV-2タンパク質をクローン化,タグ付け,発現させた.
- タンパク質とタンパク質の相互作用を特定するために,親和浄化質量スペクトロメトリーを使用した.
- ウイルス検査で検出された抗ウイルス化合物
主要な成果:
- 高信頼性のSARS-CoV-2ヒトタンパク質相互作用が332件特定されました.
- FDAが承認した薬を含む 69の化合物の標的となる 66の薬効性のあるヒトのタンパク質を発見した
- mRNAトランスレーション阻害剤とシグマ受容体の調節剤における抗ウイルス活性が発見されました.
結論:
- ホストを標的とする薬はCOVID-19の治療に有望である.
- ホスト・ファクター・ターゲティング剤を含む併用療法が有効な治療戦略である可能性があります.
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