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人間の17S U2 snRNPの分子構造

Zhenwei Zhang¹, Cindy L Will², Karl Bertram¹

¹Department of Structural Dynamics, MPI for Biophysical Chemistry, Göttingen, Germany.

|June 5, 2020

まとめ

U2小核リボヌクレオプロテイン (snRNP) 構造は,SF3B1がPRP5とTAT-SF1と相互作用し,開かれた形状を維持する方法を示しています. これは,スプライシング中に安定したU2snRNAブランチサイトヘリックス形成に必要な再配置を説明する.

科学分野:

分子生物学
構造生物学
RNA 処理

背景:

U2小核リボヌクレオプロテイン (snRNP) は,ブランチサイトアデノシンを選択することにより,前駆体mRNAのスプライシングに不可欠です.
スプライソームへの安定したU2snRNP添加には,DEAD-box ATPase PRP5が必要です.
人間のU2 snRNAの折りたたみと分岐点相互作用する幹ループ (BSL) の形成,およびSF3B1の役割は,まだ完全に理解されていません.

研究の目的:

人間の17S U2 snRNPの分子構造を決定する.
U2のsnRNP-ブランチサイト相互作用の構造的基礎を解明する.
U2 snRNPの組立と機能におけるSF3B1,PRP5,TAT-SF1の役割を理解する.

主な方法:

人間の17S U2 snRNPの3D冷凍電子顕微鏡
タンパク質のクロスリンクデータ分析
構造と生化学データを統合して分子構造を決定する.

主要な成果:

3Dの冷凍-EM構造は,SF3B1^HEATがPRP5とTAT-SF1と相互作用し,U2 snRNP内のオープンコンフォームを維持していることを示しています.
人間のU2snRNAは,PRP5,TAT-SF1とSF3B1^HEATの間にサンドイッチされたBSL構造を形成する.
安定したU2ブランチサイトヘリックス形成には,BSLの実質的な改造と相互作用するタンパク質の移動が必要である.

結論:

この研究は,安定したU2添加のために必要なTAT-SF1の構造的な説明を提供します.
PRP5によって促進された,特定されたRNPの再編成は,安定したU2-ブランチサイトの相互作用に不可欠です.
この発見は,早期のスプライソーム組立とU2 snRNP機能を制御する構造的メカニズムを明らかにする.

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