収束遺伝子は芽生えた酵母の周心体を形作る
Flora Paldi1, Bonnie Alver1, Daniel Robertson1
1The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
Nature
|June 5, 2020
まとめ
芽生えた酵母細胞は ゲノム構造と機能を構成する. 収束遺伝子とコヘシン位置付けはこれらの領域を形成し,ミトーシス中に適切な染色体分離を保証します.
科学分野:
- ゲノミクス
- 分子生物学
- 細胞生物学
背景:
- 3次元のゲノム構造は その維持,発現,伝播に不可欠です
- コヘシンタンパク質複合体は,遠隔の位置を結びつけることでゲノムを組織し,ペリセントロメアで濃縮されます.
研究 の 目的:
- 芽生えた酵母 (Saccharomyces cerevisiae) の3D構造を解明する.
- パーシントロメアゲノム組織と細胞機能,特に染色体分離との関係を確立する.
主な方法:
- Saccharomyces cerevisiaeの3D構造を調べました
- パーシントロメア構造と機能の定義における収束遺伝子の役割とコヘシンを分析した.
- ゲノム再指向がペリセントロメア組織と染色体の生物指向に与える影響を調査した.
主要な成果:
- コア・セントロメアと共にペリセントロメアの境界にある収束遺伝子は,構造と位置の凝結を定義する.
- ペリセントロメアは基底に境界遺伝子が付いているループ形をとり,マイクロチューブルの付着がこれらのループを拡張する.
- 境界遺伝子の再定位はコヘシン定位を損ね,ペリセントロメアを拡大し,染色体の生物定位を乱します.
結論:
- 遺伝子の線形配列と標的型コヘシン負荷は,有能な染色体分離のためにペリセントロメアを形作る.
- 微小管の結合は 周囲の構造を再構成する
- 3Dゲノム組織と細胞機能の間の直接的な因果関係は存在します.
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