関連する実験動画

Updated: Dec 18, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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非常に速いCRISPRの要求で

Yang Liu1, Roger S Zou2, Shuaixin He3

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD, USA. tjha@jhu.edu bwu20@jhu.edu.

Science (New York, N.Y.)
|June 13, 2020
PubMed
まとめ

私たちは光で活性化された ゲノム編集ツール CRISPR (vfCRISPR) を 急速に開発しました この方法は,DNA修復ダイナミクスの高解像度研究のためのDNA二重鎖の断絶を正確に制御します.

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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