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CRISPR01:59

CRISPR

57.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.5K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.5K
Homologous Recombination02:31

Homologous Recombination

61.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
61.9K

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Updated: Dec 18, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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非常に速いCRISPRの要求で

Yang Liu1, Roger S Zou2, Shuaixin He3

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD, USA. tjha@jhu.edu bwu20@jhu.edu.

Science (New York, N.Y.)
|June 13, 2020
PubMed
まとめ
この要約は機械生成です。

私たちは光で活性化された ゲノム編集ツール CRISPR (vfCRISPR) を 急速に開発しました この方法は,DNA修復ダイナミクスの高解像度研究のためのDNA二重鎖の断絶を正確に制御します.

さらに関連する動画

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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関連する実験動画

Last Updated: Dec 18, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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科学分野:

  • 分子生物学
  • 遺伝学
  • バイオテクノロジー

背景:

  • CRISPR-Casシステムは 強力なゲノム編集ツールです
  • DNA修復メカニズムの詳細な研究のために,DNA分裂の正確な時間的な制御が必要です.

研究 の 目的:

  • DNA修復の高解像度空間時間分析のための光誘導式CRISPR-Cas9システムを開発する.
  • DNA二重鎖の断裂修復の運動と分子現象を調査する.

主な方法:

  • 光活性化Cas9結合と分裂 (vfCRISPR) のためのケージRNA戦略を開発した.
  • DNA修復焦点 (例えば53BP1) とタンパク質動態 (例えばMRE11) を監視するために単細胞光画像を用いた.
  • DNA損傷反応のマーカーとしてH2AX酸化の拡散を測定した.

主要な成果:

  • vfCRISPRは DNAの二重鎖のインダクションを 迅速かつ同期的に 2次およびサブミクロメートルスケールで可能にします
  • MRE11 保持後の結合を含む,数分以内にDNA損傷に対する細胞反応が観察されました.
  • H2AXのリン酸化拡散と53BP1の修復焦点の動態を特徴づけた.
  • 画像による単一アレルの ゲノム操作を証明した

結論:

  • vfCRISPRは DNA修復を研究するための 前例のない空間時間解像度を提供します
  • このシステムはDNA損傷反応経路の詳細な運動分析を可能にします.
  • 精密なゲノム操作を可能にします 生物学的研究のために