アラビドプシスのストリゴラクトン信号の転写制御
Lei Wang1,2, Bing Wang3, Hong Yu1
1State Key Laboratory of Plant Genomics, and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
Nature
|June 13, 2020
まとめ
ストリゴラクトンは遺伝子転写を制御することで 植物の発達を制御する. SMXL6タンパク質は転写因子として作用し,ホルモンのシグナル伝達バランスを維持するために遺伝子を抑制します.
科学分野:
- 植物生物学
- 分子生物学
- ホルモンシグナル
背景:
- ストリゴラクトンは,成長と植物相互作用に不可欠な植物ホルモンです.
- DWARF14 (D14) やMORE AXILLARY GROWTH 2 (MAX2) のような重要なタンパク質は,ストライゴラクトンの信号伝達に関与しています.
- ストライゴラクトンによる下流転写調節は,ほとんど特徴づけられていない.
研究 の 目的:
- アラビドプシス・タリアナの ストライゴラクトン反応性遺伝子を特定するために
- ストリゴラクトン媒介による植物発達の背後にある転写メカニズムを解明する.
- ストライゴラクトンのシグナル伝達における SMXL タンパク質の役割を調査する.
主な方法:
- 合成ストリゴラクトンを利用して 応答性遺伝子をトランスクリプトミックの分析で特定しました
- 芽の枝分かれ,葉の形状,およびアントシアニンの蓄積の遺伝子調節を調査した.
- SMXL6の機能を研究するためにクロマチンの免疫沈殿とプロモーター結合測定を行った.
主要な成果:
- アラビドプシスの401のストライゴラクトン反応遺伝子を特定した.
- STRIGOLACTONEはBRANCHED 1,TCP DOMAIN PROTEIN 1,およびANTHOCYANIN PIGMENT 1遺伝子の生成を制御していることが実証されています.
- SMXL6は,SMXL6,SMXL7,SMXL8の転写を直接抑制し,自己調節された転写因子として作用する.
結論:
- ストリゴラクトンは主に下流転写を活性化して植物発育を制御する.
- SMXL6は,ストレゴラクトンの信号伝達を自己調節するDNA結合トランスクリプション因子として機能する.
- 植物における直接的な転写抑制によるホルモンシグナル制御の新しいメカニズムを明らかにした.
関連する概念動画
Cell Signaling in Plants
6.0K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.0K
Gene Regulation During Sporulation
344
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
344
Transcription
154.5K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
154.5K
Regulation of Transpiration by Stomata
30.6K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
30.6K
Regulation of Expression at Multiple Steps
1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Biological Clocks and Seasonal Responses
41.3K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.3K


