トマトの遺伝子発現と作物の改善に対する広範な構造変化の主要な影響

Michael Alonge¹, Xingang Wang², Matthias Benoit³

¹Department of Computer Science, Johns Hopkins University, Baltimore, MD 21218, USA.

Cell

|June 20, 2020

まとめ

構造変異 (SV) は作物の特徴の鍵となる. この研究ではトマトのロングリードシーケンシングを用いて何千ものSVを特定し, 遺伝子発現と重要な農業特性に幅広い影響を及ぼした.

科学分野:

ゲノミクス
植物生物学
農作物科学

背景:

構造的変種 (SV) は作物の家畜化と改良に不可欠です.
SVの全スペクトルと影響を特徴づけることは,植物遺伝学の重要な課題です.

研究の目的:

多種多様なトマト系における構造変異 (SV) を包括的に特定し,特徴づけること.
遺伝子調節,定量的な特性の変化,作物の改善におけるSVの役割を調査する.

主な方法:

100種類のトマトの長読ナノポールの配列
パンゲノム解析で14の新しい参照アセンブリが組み込まれている.
定量遺伝学とゲノム編集のアプローチ

主要な成果:

トマトパネル全体に 238,490 の構造変異 (SV) が発見されました.
遺伝子とシス調節領域に影響を及ぼす多数のSVの特定により,遺伝子発現が変化する.
SVが果物の主要な特徴 (味,サイズ,生産) と家畜化特徴 (収穫) を変化させることを示す.

結論:

構造変異 (SV) は,遺伝子型とフェノタイプの関係において,重要でしばしば過小評価される役割を果たします.
SVは作物改良と育種戦略を理解し推進するための強力なリソースです.

キーワード:

繁殖する cis 規制するコピー番号の変更謎めいた変数家畜化について投与量エピスタシスロングリードシーケンシング構造的な変化トマト

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