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関連する概念動画

ABC Transporters: Exporter01:31

ABC Transporters: Exporter

6.1K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
6.1K
ABC Transporters: Importer01:27

ABC Transporters: Importer

3.3K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
3.3K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

9.2K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
9.2K
Nuclear Export01:42

Nuclear Export

4.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
4.7K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

349
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
349
Drug Absorption Mechanism: Carrier-Mediated Membrane Transport01:19

Drug Absorption Mechanism: Carrier-Mediated Membrane Transport

5.5K
Certain large, lipid-insoluble drug molecules that resemble amino acids, peptides, or glucose, require specialized carrier proteins to facilitate their diffusion across cell membranes. This transport can occur through either facilitated diffusion, which does not require energy input, or active transport, which does require energy input.
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
5.5K

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関連する実験動画

Updated: Dec 17, 2025

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development
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Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

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原子レベルでのABCエクスポーターにおける基質トランスロケーションの捕捉

Hendrik Göddeke1, Lars V Schäfer1

  • 1Theoretical Chemistry, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, 44780 Bochum, Germany.

Journal of the American Chemical Society
|June 25, 2020
PubMed
まとめ

この研究では,ATP結合カセット (ABC) トランスポーターがどのように薬物を膜に移動させるかを視覚化するために分子ダイナミクスシミュレーションを使用した. 薬の転位メカニズムと,トランスポーターと脂質二層との基板相互作用を明らかにします.

科学分野:

  • 生物化学
  • 構造生物学
  • コンピュータ生物学

背景:

  • ATP結合カセット (ABC) トランスポーターは,ATPの水解を利用して細胞膜に基質を移動させる重要な膜タンパク質である.
  • 基板転移の原子レベルのダイナミクスを理解することは,ABCトランスポーターメカニズムを解読するために不可欠です.

研究 の 目的:

  • 原子レベルでABCトランスポーターによる基板転移のダイナミックメカニズムを解明する.
  • ダウノルビシンとヴェラパミルの輸送を,ヘテロディメア型ABC輸出者TM287/288によって調査する.

主な方法:

  • TM287/288トランスポーターを研究するために,全原子分子動力学 (MD) のシミュレーションが使用されました.
  • 高温 (375 K) で数マイクロ秒間にわたってシミュレーションを行い,ダイナミックな出来事を記録した.

主要な成果:

  • サブストラットの転位は,ABCトランスポーターの交互のアクセス形状の変化 (内向きから外向き) の後に観察されました.
  • ダウノルビシンとヴェラパミルは,異なった溶解作用により,外向きのトランスポーターから脂質二層に放出されました.
  • ヴェラパミルは2層の葉書間の動的フリップフラップを示した.

さらに関連する動画

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

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A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
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A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Published on: August 31, 2012

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関連する実験動画

Last Updated: Dec 17, 2025

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development
06:47

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Published on: August 25, 2023

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

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A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
12:25

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Published on: August 31, 2012

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結論:

  • この研究は,ABCトランスポーターの化学機械的結合と基板転位プロセスに関する原子レベルの洞察を提供します.
  • 脂質二層との差異的な基板の相互作用は,網の輸送効率に影響を与え,無益な輸送サイクルを潜在的に説明する.