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Updated: Dec 15, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

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CRISPR-dCas9を用いた端末ウリジルトランスファーゼによるサイトダイレクトアクセス

Jerrin Thomas George1, Mohd Azhar, Meghali Aich

  • 1Department of Chemistry, Indian Institute of Science Education and Research (IISER), Pune Dr. Homi Bhabha Road, Pune 411008, India.

Journal of the American Chemical Society
|July 14, 2020
PubMed
まとめ

研究者らは,合成分子を標的遺伝子に結合するために,バイオオルトゴンカル・クリック・ケミストリーを使用する新しいCRISPR技術であるsgRNA-Click (sgR-CLK) を開発した. この技術革新により 遺伝子機能の研究のための 小分子探査機と診断ツールが 精密に表示できます

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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