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Hydrogen Bonds00:26

Hydrogen Bonds

129.2K
Hydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
129.2K
Hydrogen Bonds01:04

Hydrogen Bonds

12.6K
A hydrogen bond is formed when a weakly positive hydrogen atom already bonded to one electronegative atom (for example, the oxygen in the water molecule) is attracted to another electronegative atom from another polar molecule, such as water (H2O), hydrogen fluoride (HF), or ammonia (NH3). The huge electronegativity difference between the H atom (2.1) and the atom to which it is bonded (4.0 for an F atom, 3.5 for an O atom, or 3.0 for an N atom), combined with the very small size of an H atom...
12.6K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.3K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.3K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

616
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
616
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.7K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.7K
Spin–Spin Coupling Constant: Overview01:08

Spin–Spin Coupling Constant: Overview

1.3K
In bromoethane, the three methyl protons are coupled to the two methylene protons that are three bonds away. In accordance with the n+1 rule, the signal from the methyl protons is split into three peaks with 1:2:1 relative intensities. The methylene protons appear as a quartet, with the relative intensities of 1:3:3:1.
Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must...
1.3K

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Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
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Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

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マルチプレックスH債の定量分析

Esther S Brielle1, Isaiah T Arkin2

  • 1The Alexander Grass Center for Bioengineering, Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 9190400, Israel.

Journal of the American Chemical Society
|July 22, 2020
PubMed
まとめ
この要約は機械生成です。

三つ以上のグループを含む複合水素結合は,トランスメブランヘリックスで一般的です. これらの非正規結合は,特にセリンとスレオニンを含むが,単一の水素結合よりも著しく強い.

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Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
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Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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科学分野:

  • 生物化学
  • 構造生物学
  • スペクトロスコーピー

背景:

  • バイオ分子構造と相互作用には 水素結合が不可欠です
  • 以前の研究は主に単一の水素結合に焦点を当て,マルチプレックス相互作用を無視した.
  • マルチプレックス水素結合のエネルギー学はほとんど未知のままである.

研究 の 目的:

  • マルチプレックス水素結合の普及とエネルギーについて調査する.
  • 膜経ヘリクスのセリンとスレオニン残基を含む非正規の水素結合を特徴づける.
  • タンパク質の安定性と柔軟性に対するこれらの結合の貢献を決定する.

主な方法:

  • イソトープ編集フーリエ変換赤外線 (FTIR) スペクトロスコーピー
  • 密度関数理論 (DFT) の計算
  • 超膜ヘリックス配列の分析

主要な成果:

  • 超膜ヘリクスの92%は,セリンまたはスレオニンを含む少なくとも1つの非正規の水素結合を示す.
  • これらの結合は,ヒドロキシルサイドチェーンと過剰調整されたカルボニル酸素 (位置i-4,i-3,またはi) の間で形成される.
  • 結合エンタピーは,正規の単一水素結合よりも最大127%高い.

結論:

  • セルリンとスレオニンを含む多重化水素結合は,トランスメブランヘリックスで一般的です.
  • これらの強い相互作用は,水害性環境における残留物を安定させます.
  • タンパク質の機能に不可欠な 柔軟性を提供します