Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Gastrulation01:56

Gastrulation

64.8K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
64.8K
Master Transcription Regulators02:23

Master Transcription Regulators

7.5K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.5K
Epigenetic Regulation01:46

Epigenetic Regulation

33.2K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.2K
Epigenetic Regulation01:37

Epigenetic Regulation

3.6K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.5K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.5K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.6K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

metilene<sup>3</sup>: identifying DMRs across multiple conditions with auto-classification.

Nature communications·2026
Same author

DNAviWEB: sequencing-free clinical screening of liquid biopsies.

Nucleic acids research·2026
Same author

Comprehensive Lineage Tracing Maps the Landscape of Cell Fate Decisions in Mouse Embryogenesis.

bioRxiv : the preprint server for biology·2026
Same author

Chronic lymphocytic leukemia with <i>IGH</i>::<i>BCL3</i>-translocation is characterized by a homogeneous and distinct genetic and epigenetic landscape.

HemaSphere·2026
Same author

The spectrum of immunoglobulin heavy chain enhancer hijacking in chronic lymphocytic leukemia.

Leukemia·2026
Same author

scGeno: a Hidden Markov Model approach to denoise chromosome-scale genotypes from single-cell data.

Bioinformatics advances·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: Dec 13, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

1.5K

マウスガストルレーションによる表遺伝子調節機能

Stefanie Grosswendt1, Helene Kretzmer1, Zachary D Smith2,3,4

  • 1Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany.

Nature
|July 31, 2020
PubMed
まとめ
この要約は機械生成です。

この研究では,ポリカンブ抑制複合体2 (PRC2) がマウスの早期発達の過程で生殖系統の運命を制限する上で重要な役割を果たしていることが明らかになりました. PRCの変異性フェノタイプは ガストゥルレーション前に現れ,細胞運命を決定する新たな洞察を提供します.

さらに関連する動画

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.8K
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.4K

関連する実験動画

Last Updated: Dec 13, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

1.5K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.8K
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.4K

科学分野:

  • 発達生物学
  • エピジェネティクス
  • ゲノミクス

背景:

  • 発達中の細胞運命を決定することは,転写因子と表遺伝子機構に依存している.
  • これらのレギュレータの正確な発達的役割を理解することは,複雑な変異性現象型のために困難です.
  • 単細胞RNA配列解析は 初期胚の発達の研究を進めてきました

研究 の 目的:

  • 初期のマウス胚形成における重要な発達調節体の役割を調査する.
  • 細胞運命を制限するポリコンブ抑制複合体 (PRC1とPRC2) の機能を分析する.
  • 複数のミュータントマウス胚を同時に測定するためのプラットフォームを開発する.

主な方法:

  • シングルセルRNAの配列化プラットフォームを用いた.
  • 複数の突然変異のマウス胚を同時に 形態学的および転写的データのために検査した.
  • PRC1とPRC2を含む10の重要な発達調節体に焦点を当てています.

主要な成果:

  • PRC1とPRC2の間の実質的な協力性が特定され,PRC2は生殖系制限において支配的な役割を果たしている.
  • PRCの変異性フェノタイプは,早期の表遺伝的および転写的変化に伴い,ガストルレーション前に発現することが観察されました.
  • 10つの重要なレギュレータで 強力な形態学的および転写情報を生成した.

結論:

  • PRC2はマウスの発達初期に生殖細胞の運命を制限する上で重要な役割を果たします.
  • PRC媒介による発達効果はガストルレーションに先行する.
  • 開発された実験的枠組みは,単一の細胞から発生する細胞多様性の定量分析を可能にします.