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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

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Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Inheritance of Chromatin Structures03:17

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Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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Combinatorial Gene Control02:33

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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記憶 の 配列 解析 は,特定 の 細胞 の 行動 と 関連 し た 遺伝 的 な 単細胞 遺伝子 表達 プログラム を 明らかに し ます

Sydney M Shaffer1, Benjamin L Emert2, Raúl A Reyes Hueros3

  • 1Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA, USA.

Cell
|August 1, 2020
PubMed
まとめ

細胞の遺伝子発現の変動は 細胞分裂を超えて持続し 生物学的記憶の形態を明らかにします この研究は,世代を超えて持続的な非遺伝的発現変化を持つ遺伝子を識別するためにMemorySeqを導入します.

キーワード:
がん薬剤耐性遺伝子発現記憶シングルセル

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Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Last Updated: Dec 13, 2025

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Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq
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Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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科学分野:

  • 細胞生物学
  • ゲノミクス
  • エピジェネティクス

背景:

  • 個々の細胞は,非遺伝的要因により,著しい遺伝子発現の多様性を表している.
  • これらの遺伝子発現の変動の持続時間は,特に単細胞分裂を超えて,よく理解されていません.
  • 既存の単細胞遺伝子発現測定技術は単一の時間点に限られており,細胞記憶の研究を妨げています.

研究 の 目的:

  • 単細胞における非遺伝的遺伝子発現の変動が複数の細胞分裂に持続するかどうかを調査する.
  • 長期にわたる非遺伝的発現変化を持つ遺伝子を特定するための新しい方法を開発し,適用する.
  • 細胞の記憶の 生物学的意味を探るため

主な方法:

  • ルリアとデルブリュックの変動分析と 集団ベースのRNA配列解析を組み合わせた.
  • 複数の細胞分裂にわたって持続するトランスクリプトーム全体の遺伝子変動を識別するために,MemorySeqと呼ばれる方法を開発し,利用した.
  • 同質のクローン集団内の希少な細胞サブ集団を分析した.

主要な成果:

  • 複製集団内の希少な細胞で調整された発現を示す複数の遺伝子モジュールを特定した.
  • これらの稀な亜集団は,抗癌療法中の増殖を含む,独特の生物学的行動を示した.
  • 非遺伝的,多世代間の遺伝子発現の変動を定量的に測定できることを示した.

結論:

  • 非遺伝的要因は,複数の細胞分裂にわたって持続する遺伝性の細胞状態を誘導し,生物学的記憶の形態を表します.
  • MemorySeq方法は,非遺伝的細胞記憶に関与する遺伝子の識別と特徴付けを可能にします.
  • この発見は,細胞状態の非遺伝的遺伝性は,病気と治療に対する潜在的な影響を持つ量化可能な生物学的性質であることを示唆しています.