E. coli の13キロダルトンのトウモロコシミトコンドリアタンパク質は,バイポラリス・メイディス毒素に対する感受性を与える
R E Dewey1, J N Siedow, D H Timothy
1Department of Crop Science, North Carolina State University, Raleigh 27695.
Science (New York, N.Y.)
|January 15, 1988
まとめ
テキサスのトウモロコシの男性無菌性サイトプラズマ (cms-T) は,ユニークなミトコンドリア遺伝子に関連しています. この遺伝子は13kDaのタンパク質を生産し,BmT-毒素とメトミールに対する感受性を与え,男性の不妊症を説明します.
科学分野:
- 植物生物学 植物生物学
- ミトコンドリア遺伝学 ミトコンドリア遺伝学
- バイオケミストリー バイオケミストリー
背景:
- テキサスのトウモロコシの男性不妊性サイトプラズマ (cms-T) は,サイトプラズマ的に受け継がれる男性不妊性によって特徴付けられています.
- cms-Tトウモロコシのミトコンドリアは,Bipolaris maydisからのBmT毒素と殺虫剤メトミールに特異的に敏感である.
研究 の 目的:
- cms-Tトウモロコシにおけるユニークなミトコンドリア遺伝子の役割を調査する.
- 男性の不妊症に関連する13キロダルトンポリペプチドの機能を決定する.
主な方法:
- Escherichia coli.におけるcms-T特異的なミトコンドリア遺伝子の発現.
- BmT-毒素またはメトミールを加えた後にE. coli培地における全細胞呼吸と球体プラストの腫れを測定する.
主要な成果:
- 13kDaポリペプチドを生成するE. coliは,BmT毒素またはメトミールに曝露されたときに,呼吸が抑制され,球体プラストの腫れを示した.
- これらの効果は,孤立したcms-Tミトコンドリアで観察されたものを模倣した.
- 13 kDa のポリペプチドのアミノ末端領域は,毒素相互作用において決定的であると特定されました.
結論:
- 13キロダルトンのポリペプチドは,cms-Tトウモロコシのミトコンドリアに毒素の感受性を与えるのに関与しています.
- このタンパク質は,cms-Tトウモロコシで見られる男性不妊症のフェノタイプに重要な役割を果たしています.
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