理性 的 に 設計 さ れ た クープレドキシン の 中 で 赤 緑 青 の 色彩 スペクトル を 横断 する
Karl J Koebke1, Victor Sosa Alfaro1, Tyler B J Pinter1
1Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
Journal of the American Chemical Society
|August 14, 2020
まとめ
研究者らは新しい構造で 独立した青銅のタンパク質を作りました この発見により 赤,緑,青の銅の部位が 固有のコプレドキシン折り畳みの外で 調べられるようになりました
科学分野:
- 生物化学
- タンパク質工学
- バイオ有機化学
背景:
- 青銅のタンパク質は独特のCu (II) 幾何学を持ち,ネイティブのクプレドキシン構造の外では複製することが困難である.
- 以前の設計では,外部リガンドで青に調節可能な緑の銅タンパク質を達成しましたが,新しい青銅部位は難解でした.
- 螺旋状のバンドルに以前設計された赤い銅タンパク質サイトは,ニトロシアニン模倣体 (CuHis2CysGlu) として特定されました.
研究 の 目的:
- 赤,緑,青の銅色染色体を 形成する能力のある新型構造を 合理的に設計する
- カップレドキシン折りたたみとは独立した銅部位の構造的・機能的差異を調査する.
- ネイティブ・クプレドキシンからの変異戦略をデノボのタンパク質設計に適応させる.
主な方法:
- 赤い銅の場所のために以前設計された3つの螺旋の束のエスカファードを使用しました.
- 金属結合部位の回転と軸性メチオニンの除去を含む合理的な設計と変異戦略を採用した.
- 発現した銅タンパク質の部位を,そのスペクトル特性 (赤,緑,青) で特徴づけた.
主要な成果:
- 金属の結合部位を回転させることで 緑色の銅のタンパク質を成功させた
- 青銅タンパク質を青銅タンパク質に変換し,軸性メチオニンを除去した.
- 赤,緑,青の銅タンパク質が 同じアルファ・ヘリクルス・スキャフォルドで作られる
結論:
- アルファ螺旋折れの中で赤,緑,青の銅部位を成功裏に設計した.
- 赤,緑,青の銅タンパク質の構造-機能関係が,クプレドキシン折れの外で初めて直接比較された.
- 特殊なスペクトルおよび機能特性を備えたメタロプロテインを設計するための新しい道を開きました.
さらに関連する動画
07:11ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
Published on: August 19, 2021
2.9K
11:26Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
Published on: September 12, 2014
12.9K
関連する概念動画
01:24Color Vision
1.2K
Color perception begins in the retina, the light-sensitive layer at the back of the eye. Two main theories explain how colors are seen: the trichromatic theory and the opponent-process theory. The trichromatic theory, proposed by Thomas Young in 1802 and extended by Hermann von Helmholtz in 1852, suggests that color vision is based on three types of cone receptors in the retina. These cones are sensitive to different but overlapping ranges of wavelengths corresponding to red, blue, and green.
1.2K
01:22Photoreceptors and Visual Pathways
8.3K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
8.3K
03:02Colors and Magnetism
13.5K
Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
13.5K
01:29The Photochemical Reaction Center
5.0K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
5.0K
01:24Redox Reactions
57.9K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
57.9K
01:11Channel Rhodopsins
3.0K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
3.0K