酵母とヒトのCCAAT結合タンパク質には,機能的に互換性のある異質なサブユニットがあります
L A Chodosh1, J Olesen, S Hahn
1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Cell
|April 8, 1988
まとめ
酵母とヒトのCCAAT結合タンパク質であるHAP2/HAP3とCP1は,DNA認識の特性で類似しています. 驚くべきことに,それらのサブユニットは,機能的に互換性があり,CCAAT要素を依然として結合するハイブリッド複合体を形成します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- S. cerevisiaeのHAP2およびHAP3遺伝子産物は,CCAAT要素を認識する複雑な構造を形成する.
- 人間の細胞には,CP1を含む複数のCCAAT結合タンパク質があり,それらはヘテロマーとして機能します.
研究 の 目的:
- 酵母HAP2/HAP3とヒトのCP1 CCAAT結合複合体の機能的および構造的類似性を調査する.
- 酵母とヒトのCCAAT結合タンパク質のサブユニット互換性の可能性を調査する.
主な方法:
- 酵母HAP2/HAP3とヒトCP1.1のDNA結合特性の比較分析
- DNA配列認識とDNAコンタクトポイントの調査.
- ハイブリッド酵母-ヒトCCAAT結合複合体の機能評価.
主要な成果:
- 人間のCP1と酵母HAP2/HAP3は,実質的に同一のDNA結合特性と標的配列認識を示しています.
- この2つの複合体は,CCAAT要素の内部で同様のDNA接触をします.
- CCAAT要素の変異は,両方の複合体に同様の影響を及ぼします.
- 酵母と人間のサブユニットは機能的に互換性があり,活性ハイブリッド複合体を形成します.
結論:
- 酵母とヒトのCCAAT結合タンパク質は,高度に保存されたDNA認識機構を共有しています.
- サブユニットの機能的互換性は,CCAAT要素認識のための保存された進化的起源と構造的基礎を示唆しています.
- これらの発見は,種間の遺伝子調節の基本的メカニズムについての洞察を提供します.
関連する概念動画
The Central Dogma
Overview
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Ligand Binding and Linkage
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Allosteric Proteins-ATCase
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...


