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Protein Folding01:22

Protein Folding

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Overview
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Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

4.9K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

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Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
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Updated: Dec 11, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
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Chemical Dimerization-Induced Protein Condensates on Telomeres

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ディメリゼーションの品質管理の構造的基礎

Elijah L Mena1,2, Predrag Jevtić1,3, Basil J Greber4,5

  • 1Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA, USA.

Nature
|August 21, 2020
PubMed
まとめ
この要約は機械生成です。

この研究では,SCF-FBXL17による品質管理が,欠陥のあるタンパク質ダイマーを識別し,分解する方法を明らかにした. 不活性なBTBタンパク質ヘテロダイマーを標的とし,その構造を破壊し,プロテオスタシスを確保します.

さらに関連する動画

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

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Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
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Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

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関連する実験動画

Last Updated: Dec 11, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

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Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

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科学分野:

  • 分子生物学
  • 構造生物学
  • セルラー品質管理

背景:

  • タンパク質の品質管理経路は 誤った折りたたみのタンパク質を排除することで 神経変性疾患を予防する上で極めて重要です
  • ディメリゼーションの品質管理は,誤ったサブユニットを持つタンパク質複合体を除去することによってプロテオスタシスを強化しますが,検出メカニズムは不明です.

研究 の 目的:

  • SCF-FBXL17 E3リガスが異常タンパク質ジマーを標的と分解する構造的メカニズムを解明する.
  • SCF-FBXL17がBTBタンパク質の機能性ホモディマーと不活性ヘテロディマーを区別する方法を理解する.

主な方法:

  • BTBタンパク質ジマーと相互作用するSCF-FBXL17の構造分析.
  • タンパク質複合体の普遍化と分解を評価する生化学的測定法
  • ダイマー安定性における分子間βシート形成の役割を調査する.

主要な成果:

  • SCF-FBXL17は,特に不活性なBTBタンパク質ヘテロダイマーを分解する.
  • 安定した分子間βシートがない異常ジメルは,SCF-FBXL17によって認識され,破壊される.
  • E3リガゼは,複合的な解離後に単一のBTBドメインに結合し,汎用化につながります.

結論:

  • SCF-FBXL17は,BTBドメインの形状と互補性を検知し,正しい複合組成を確保するメカニズムを使用しています.
  • この品質管理メカニズムは,プロテオスタシスを維持し,非機能的なタンパク質複合体の蓄積を防ぐために不可欠です.