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関連する概念動画

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Stem Cell Niche01:26

Stem Cell Niche

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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Multipotency and Niche of Bulge Stem Cell01:06

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A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
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Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

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Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Updated: Nov 11, 2025

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
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ヘテロタイプの細胞間通信は,腺幹細胞の多効性を調節する.

Alessia Centonze1, Shuheng Lin1, Elisavet Tika1

  • 1Laboratory of Stem Cells and Cancer, Université Libre de Bruxelles (ULB), Brussels, Belgium.

Nature
|August 28, 2020
PubMed
まとめ

ルミナル細胞 (LCs) は通常,腺における基礎幹細胞 (BSCs) の多効性を抑制する. LCの除去により,BSCは胚のような多能性を取り戻し,幹細胞のアイデンティティを維持するための重要なコミュニケーション経路を明らかにします.

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Last Updated: Nov 11, 2025

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Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
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科学分野:

  • 幹細胞生物学
  • エピテリア生物学
  • セル・シグナル

背景:

  • 乳腺および前立腺のような腺上皮は,基礎細胞 (BCs) と光細胞 (LCs) で構成されています.
  • 大人の基礎幹細胞 (BSC) は典型的には単能であるが,再生または腫瘍遺伝子の活性化中に多能性を回復することができる.
  • 正常な生理学的条件下でBSCの多効性を制限するメカニズムは現在不明です.

研究 の 目的:

  • 基底幹細胞 (BSC) の多効性を制限するメカニズムを調査する.
  • 血統の忠誠を保つのに関与する 細胞と分子プレーヤーを特定する

主な方法:

  • 光細胞 (LC) 剥離後のBSC多効性 in vivo (マウス) と in vitro (オルガノイド) で調査した.
  • BSCにおける遺伝子発現変化を分析するために,大量および単細胞RNA配列を解析した.
  • シグナル伝達経路を特定するための単細胞データからのリガンド受容体相互作用の予測.

主要な成果:

  • LCの切除により,BSCのマルチポテンシーが再活性化され,ハイブリッドの基礎細胞と光細胞の分化プログラムが誘発された.
  • LCsによって分泌される腫瘍死因 (TNF) は,BSC多効性の主要な阻害剤として特定されました.
  • ノッチ,Wnt,EGFR経路はLCの切除時に活性化され,その抑制またはTNFの刺激はBSCの多効性を阻害した.

結論:

  • LC と BC の間の異型通信は,腺上皮質幹細胞の系統忠節性を維持するために不可欠です.
  • LCsからのTNFシグナリングは,正常な生理学的条件下でBSCの多効性を積極的に抑制する.
  • このコミュニケーションを理解することで 幹細胞の調節と潜在的な治療目標の洞察が得られます