ELF3のプリオンのようなドメインは,アラビドプシスの熱センサーとして機能する
Jae-Hoon Jung1,2, Antonio D Barbosa1, Stephanie Hutin3
1Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Nature
|August 28, 2020
まとめ
植物は,ポリグルタミン (polyQ) の繰り返し長さが熱応答性を決定する夜間複合タンパク質ELF3を通して温度を感知します. このタンパク質は温度による 急速な相変化を経験し 新しい植物熱感メカニズムを明らかにします
科学分野:
- 植物生物学
- 分子生物学
- バイオ物理学
背景:
- 植物の成長と発達に影響を与える重要な要因は気温であり,気候変動は植物の現象学に影響する.
- 植物が温度を感知する 分子機構は ほとんど不明です
- ELF3,ELF4,およびLUXを含む夜間複合体は,植物生理時計の重要な構成要素であり,温度に対応する転写抑制剤として作用する.
研究 の 目的:
- 植物における温度感知の基礎となる分子メカニズムを解明する.
- EARLY FLOWERING 3 (ELF3) タンパク質,特にそのポリグルタミン (polyQ) リピートおよびプリオンのようなドメイン (PrD) の熱応答性における役割を調査する.
主な方法:
- 異なる気候の植物のELF3ポリQ繰り返しの長さの分析
- ELF3 PrD 断片の in vitro 段階移行試験を含む生化学的および生体学的測定
- 温度によるELF3局所化 (斑点形成) の変化を観察する顕微鏡検査 in vivo
主要な成果:
- ELF3におけるポリQリピートの長さは,その熱応答性と相関する.
- より暑い気候の植物のELF3タンパク質は,熱反応性が低下し,検出可能なPrDがない.
- 温度により,ELF3の急速で可逆的な相変化 (斑点形成) が生体内で発生し,PrDによってPrDの液滴形成が生体内で発生する.
結論:
- ELF3のプリオンのようなドメインは,温度感知能力にとって極めて重要です.
- ELF3の温度誘発による液体-液体相分離は,植物における熱感知のための新しいメカニズムを表しています.
- このメカニズムは,温度変動に対する植物の反応の急速な調整を可能にします.
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