ケラチンは,哺乳類の胚で不対称に遺伝する運命決定因子である

Hui Yi Grace Lim¹, Yanina D Alvarez¹, Maxime Gasnier¹

¹Institute of Molecular and Cell Biology, ASTAR, Singapore, Singapore.

Nature

|August 28, 2020

まとめ

ケラチン中間繊維は早期哺乳類の胚の細胞運命を決定する. トロフェクトダームの特異化と胎盤の形成を促し,非対称的に遺伝する.

科学分野:

発達生物学
細胞生物学
分子生物学

背景:

哺乳類の胚の発達には,内部の細胞質 (胎児) とトロフェクトダーム (胎盤) の系統の特定が含まれます.
運命の決定因子の非対称的な遺伝は,多くの生物の系統特異を駆り立てるが,哺乳類の早期の発達ではない.

研究の目的:

哺乳類の胚の早期発達における中間線維,特にケラチンの役割を調査する.
ケラチンが哺乳類の胚で非対称的に遺伝する運命決定因子として機能するかどうかを判断する.

主な方法:

細胞分裂中のケラチンの遺伝を観察するための生胚イメージング.
BAF染色体再構成複合体の異質性によって引き起こされるケラチン変性の分析.
YAP依存のCDX2発現とケラチン局所化との関連を調査する.

主要な成果:

ケラチンは,BAF複合体によって影響される細胞間変動性を示す最初の主要な細胞骨格成分です.
ケラチンは分裂時に外部の子細胞によって非対称的に受け継がれます.
非対称なケラチン遺伝は皮質を安定させ,アピカル極化を促進し,YAP依存のCDX2発現を誘導し,トロフェクトダーム細胞を特定する.

結論:

ケラチンは哺乳類の胚で不対称に遺伝する運命決定因子として機能する.
ケラチン調節における細胞間異質性は,トロフェクトドーム分離前の系統運命を影響する.
この研究は,哺乳類の発達における中間フィラメントの早期の役割を特定しています.

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