レウシンに富んだリピート受容体のようなキナーゼによるキノン感知
Anuphon Laohavisit1, Takanori Wakatake1,2, Nobuaki Ishihama1
1RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Nature
|September 4, 2020
まとめ
植物は,CARD1受容体キナーゼを含むカルシウムシグナル伝達経路を通じてキノンを感知する. この発見は 植物免疫と 寄生虫の発達に 光を当てました
科学分野:
- 植物生物学
- 分子信号
- 生物化学
背景:
- キノンは全ての生命体に見られる 重要な分子です
- 植物,特に非寄生生物のシグナル伝達作用は十分に理解されていません.
- DMBQのようなキノンが寄生植物-ハウストリウム開発に関与することが知られている.
研究 の 目的:
- アラビドプシス・タリアナのキノン (DMBQ) 検出メカニズムを調査する.
- キノンの信号伝達に関与する植物受容体を特定する.
- 植物免疫と寄生植物発達のキノンのシグナル伝達の役割を調査する.
主な方法:
- アラビドプシスの遺伝子スクリーニングを進めて,DMBQに反応しない変異体を分離する (カード1).
- レウシンに富んだリピート受容体型のキナーゼをコードする CARD1 遺伝子の特徴.
- 野生型およびカード1変異植物におけるDMBQ誘発カルシウムシグナル伝達および防御遺伝子発現の分析.
- 寄生植物Phtheirospermum japonicumのCARD1同種を用いた補足研究
主要な成果:
- アラビドプシスのDMBQシグナル伝達は,細胞溶液Ca2+上昇によって発生する.
- CARD1遺伝子は DMBQの知覚と植物免疫に不可欠です
- バクテリアの病原体に対する防御反応が低下している.
- DMBQシグナル伝達とCARD1は,寄生植物P. japonicumのハウストリウム発育に不可欠である.
結論:
- 植物では,動物や細菌とは異なるキノンのシグナル伝達のために,レウシンに富んだリピート受容体のようなキナーゼ (CARD1のような) を利用する.
- CARD1は,寄生植物と非寄生植物の両方でDMBQ誘発カルシウムシグナル伝達を媒介する保守的な役割を果たします.
- この研究は,キノンのシグナル伝達が植物免疫と寄生虫の植物と宿主の相互作用における新たな役割を明らかにしている.
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