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関連する概念動画

Rab Proteins01:14

Rab Proteins

4.8K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.8K
Adrenergic Receptors: β Subtype01:26

Adrenergic Receptors: β Subtype

3.2K
β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
3.2K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.3K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.3K
Aquaporins01:25

Aquaporins

5.9K
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
5.9K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.7K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.7K
Rab Cascades01:25

Rab Cascades

3.3K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.3K

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関連する実験動画

Updated: Dec 9, 2025

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
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Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

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スナップショット: β-アレスティン機能

Seungkirl Ahn1, Sudha K Shenoy2, Louis M Luttrell3

  • 1Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

Cell
|September 5, 2020
PubMed
まとめ

アレスティンは,Gタンパク質結合受容体によって開始される信号経路に関与する重要なアダプタータンパク質です. これらのタンパク質は,受容体-Gタンパク質の相互作用を阻害するだけでなく,様々な細胞機能に影響を与え,ダイナミックな役割を果たします.

さらに関連する動画

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
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Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

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関連する実験動画

Last Updated: Dec 9, 2025

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
08:21

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

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Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
09:03

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

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科学分野:

  • 細胞生物学
  • 分子信号
  • 生物化学

背景:

  • アレスティンは,細胞に普遍的に発現するアダプタータンパク質です.
  • 7トランスメブランGタンパク質結合受容体 (GPCRs) と相互作用することが知られている.
  • Gタンパク質の結合を阻害することでGPCRを無感化させる役割のために最初に特定されました.

研究 の 目的:

  • アレスティンの機能の進化を強調する.
  • アレストインのダイナミックなタンパク質の相互作用を強調する.
  • GPCRシグナル伝達を超えたアストレインの 細胞の役割の拡大を探求する.

主な方法:

  • アレステンの研究に関する文献レビュー
  • タンパク質とタンパク質の相互作用の研究の分析
  • 信号経路の構成要素の調査

主要な成果:

  • アレスティンは多種多様な膜伝達カスケードに参加する.
  • アレスティンは様々なダイナミックなタンパク質の相互作用を示します.
  • アレスティンはGPCR無敏感化を超えて複数の細胞機能を調節する.

結論:

  • アレスティンは,細胞シグナル伝達において多面的な役割を担う,多用途のアダプタタンパク質です.
  • アレスティンの機能的なレパートリーは絶えず拡大しています.
  • アレスティンの動態を理解することは 複雑な細胞プロセスを解読する鍵です