酵母細胞型特異の抑制剤アルファ2は,細胞型特異でないタンパク質と協力して作用する

C A Keleher¹, C Goutte, A D Johnson

¹Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

Cell

|June 17, 1988

まとめ

GRMという新しいタンパク質は,酵母アルファ2抑制体と協力的に結合する. この相互作用は,遺伝子抑制に不可欠であり,GRMがオペレータセンターとアルファ2ダイマーが端を結合することを含む.

科学分野:

分子生物学は分子生物学である.
イースト遺伝学イースト遺伝学
トランスクリプションに関する規則

背景:

酵母アルファ2タンパク質は,細胞型特異の転写抑制剤として機能する.
アルファ2タンパク質は,標的遺伝子の上流にある特定のオペレーター配列に結合することによってその機能を果たします.

研究の目的:

酵母アルファ2タンパク質と相互作用する新しいタンパク質を特定し,特徴づけること.
協同結合のメカニズムと,転写抑制におけるその役割を解明する.

主な方法:

酵母遺伝学を用いたタンパク質-タンパク質相互作用の研究.
DNA認識配列を決定するために変異したオペレータの分析.
テストプロモーターを用いたインビボ抑制アッセイ.

主要な成果:

GRMと呼ばれる新しいタンパク質が識別され,アルファ2と協力的に結合する.
GRMはオペレータの中心部と結合し,α2ジメルはオペレータの末端と結合し,ユニークなタンパク質配列を作成します.
GRMとα2認識シーケンスの両方が,in vivo抑制に不可欠です.

結論:

GRMとalpha2は,タンパク質とタンパク質の相互作用によって媒介されるオペレータでの協力結合を示します.
アルファ2のN端領域は,GRMとの協同結合に関与しています.
この相互作用は,GRM/alpha 2複合体によって媒介される転写抑制に極めて重要です.

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