C. elegansは,病原性の回避を学ぶために,細菌の非コーディングRNAを解釈する
Rachel Kaletsky1,2, Rebecca S Moore1, Geoffrey D Vrla1
1Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Nature
|September 10, 2020
まとめ
病原菌への単発暴露
科学分野:
- 微生物学
- 遺伝学
- 神経科学
背景:
- 食物と病原体を区別しなければならない
- バクテリアの接触は 虫の行動に影響を与えます
研究 の 目的:
- 病原体回避学習における小さなRNAの役割を調査する.
- 世代を超えた回避行動の遺伝的メカニズムを決定する.
主な方法:
- 病原性Pseudomonas aeruginosa (PA14) 小型RNAに対するC. elegansの暴露
- 治療された虫やその後の世代における回避行動の評価
- RNA干渉 (RNAi),PIWIと相互作用するRNA (piRNA) 経路,生殖系,ASIニューロンの関与を調査する.
- 特定のバクテリアの非コーディングRNAとその標的であるC. elegansを特定する.
主要な成果:
- PA14小RNAへの単発暴露は,C. elegansにおける病原体回避を誘導した.
- この学ばれた回避は 4世代に渡って受け継がれました
- RNAiとpiRNA経路,生殖線,ASIニューロンは誘発回避と遺伝の両方に不可欠でした.
- 特定のPseudomonas aeruginosa非コーディングRNA (P11) は,学習された回避のために必要で十分であった.
- この回避行動に必要な標的としてC.elegans遺伝子maco-1が特定されました.
結論:
- 小型RNAは,C. elegansにおける学習された病原体回避を媒介する.
- この学習された行動とその基礎となる 分子機構は 世代を超えて受け継がれます
- 非コーディングRNA依存メカニズムは 虫が微生物の脅威を世代を超えて感知し 情報を伝達することを可能にします
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