Xenopusのcdc2タンパク質は,ミトーシスの細胞質調節体であるMPFの構成要素である
W G Dunphy1, L Brizuela, D Beach
1Department of Biology, University of California, San Diego, La Jolla 92093.
Cell
|July 29, 1988
まとめ
酵母 Suc1遺伝子産物 (p13) は,Xenopusの成熟促進因子 (MPF) に結合することで,Xenopusの成熟促進因子を抑制する. これは,p13がXenopus cdc2タンパク質を含むMPF成分と相互作用することを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- クセノプスの成熟促進因子 (MPF) はミトスのエントリーを誘導する.
- cdc2キナーゼは,分裂酵母におけるミトス発起を調節する.
- 酵母 suc1 遺伝子産物 (p13) は,cdc2 キナーゼと相互作用する.
研究 の 目的:
- 酵母 Suc1遺伝子産物 (p13) がXenopusの卵エキスのMPFに及ぼす抑制効果を調査する.
- p13がMPFの活動を抑制するメカニズムを決定する.
主な方法:
- Xenopusの卵から細胞のない抽出物を利用した.
- 相互作用するタンパク質を分離するためにp13親和染色を用いた.
- p13.への反応として測定されたMPF活性.
主要な成果:
- 酵母p13は,XenopusエキスのMPF活動を強力に抑制しました.
- p13は,MPFに直接結合し,MPF活動の定量的な枯渇につながります.
- Xenopus cdc2タンパク質と42 kdタンパク質は,p13.に結合するMPF成分として特定されました.
結論:
- 酵母 suc1遺伝子産物 (p13) は,Xenopus MPF.の阻害剤として作用する.
- Xenopus cdc2タンパク質と42 kdタンパク質は,MPFの成分である可能性が高い.
- これらの発見は,酵母とXenopus.の間の細胞周期調節における保存されたメカニズムを強調しています.
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