関連する実験動画
Updated: Jul 11, 2026

11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
ergは,染色体21のヒトETS関連遺伝子で,代替スプライシング,ポリアデニレーション,およびトランスレーションが用いられる
まとめ
erg遺伝子は,代替スプライシングとトランスレーションイニシエーションを通じて,2つの異なるタンパク質を生成します. このメカニズムは,単一のerg遺伝子が関連するポリペプチドを生成し,細胞のプロセスに影響を与えることを可能にします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 腫瘍学 腫瘍学
背景:
- E26鳥類白血病ウイルスはv-mybとv-etsの腫瘍遺伝子を含んでいる.
- v-etsに関連したerg遺伝子は,ヒトのCOLO320細胞で特定されました.
研究 の 目的:
- 代替的にスプライスされたergトランスクリプトの核酸配列を決定するには,erg 2.
- erg遺伝子産物の発現と局所化を調査する.
主な方法:
- ergトランスクリプトの核酸配列解析.
- 補完的なDNAのインビトロ発現.
- 遺伝子局所化のためのソマティック細胞遺伝分析.
主要な成果:
- erg 2は,コード化フレームシフトと99アミノ酸挿入を引き起こすスプライシングイベントによって,erg 1と異なる.
- 試験管内発現では41キロダルトンと52キロダルトンのポリペプチドが得られ,代替翻訳開始が示唆された.
- erg遺伝子は,ヒト染色体21に局所化された.
結論:
- 代替のスプライシングとトランスレーション開始部位により,単一の遺伝子から2つの関連するエルグポリペプチドの合成が可能になります.
- これらのメカニズムは,erg遺伝子製品の機能的多様性に寄与する.
関連する概念動画
What is Gene Expression?
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

