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Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

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Updated: Jul 7, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

イーストオペレーターは,上流の活性化部位を重なり合わせている.

J W Kronstad, J A Holly, V L MacKay

    Cell
    |July 31, 1987
    PubMed
    まとめ
    この要約は機械生成です。

    酵母A細胞のBAR1遺伝子産物は,アルファファクターを無効化する. その転写はMATα2タンパク質とα因子によって調節され,酵母交配と遺伝子調節に影響を与えます.

    さらに関連する動画

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
    08:48

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

    Published on: January 26, 2017

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
    07:18

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

    Published on: May 15, 2018

    関連する実験動画

    Last Updated: Jul 7, 2026

    Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
    09:22

    Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

    Published on: November 26, 2013

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
    08:48

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

    Published on: January 26, 2017

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
    07:18

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

    Published on: May 15, 2018

    科学分野:

    • 分子生物学は分子生物学である.
    • イースト遺伝学 イースト遺伝学
    • 遺伝子規制 遺伝子規制

    背景:

    • Saccharomyces cerevisiae の BAR1 遺伝子は,a 細胞のみで発現し,α 細胞によって生成される交配フェロモンであるアルファファクターを不活性化するタンパク質をコードします.
    • イーストの交配における重要な調節体であるMATα2タンパク質は,アルファおよびa/alpha二倍体細胞におけるBAR1を含むa細胞特異遺伝子を抑制する.
    • A細胞のBAR1転写は,アルファ因子に曝露すると誘発され,複雑な規制ネットワークを示します.

    研究 の 目的:

    • 菌糸体Saccharomyces cerevisiaeにおけるBAR1遺伝子発現を制御する規制メカニズムを調査する.
    • MATアルファ2媒介による抑制とアルファ因子誘発によるBAR1転写の活性化に起因する特定のDNA配列を特定する.
    • 酵母における遺伝子転写の負の制御に関するこれらの発見の意味を理解するために.

    主な方法:

    • BAR1遺伝子の5'ノンコーディング領域の削除分析.
    • 特定の交配因子と調節タンパク質への反応として,遺伝子転写の分析.

    主要な成果:

    • 削除分析により,BAR1の主要な上流活性化部位 (UAS) が,MATアルファ2抑制に不可欠な31bpのオペレーター配列と重なり合っていることが明らかになった.
    • 配列TGAAACAは,BAR1.1のアルファ因子刺激トランスクリプションを媒介するものとして特定されました.
    • これらの発見は,酵母における遺伝子発現の制御に不可欠な規制の重複を強調しています.

    結論:

    • この研究は,MATアルファ2による抑制とアルファ因子による活性化の両方を含むBAR1遺伝子発現の複雑な調節を解明しています.
    • 抑制と活性化の重複する規制要素は,酵母における負の転写制御を理解する上で重要な意味を持つ.
    • 特定されたTGAAACA配列は,A細胞におけるフェロモン誘発遺伝子発現の重要な媒介である.