Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Mismatch Repair01:36

Mismatch Repair

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Peripheral inflammatory cytokines and motor symptoms in persons with Parkinson's disease.

Brain, behavior, & immunity - health·2022
Same author

The validity of surface EMG of extra-diaphragmatic muscles in assessing respiratory responses during mechanical ventilation: A systematic review.

Pulmonology·2020
Same author

Benzyloxycarbonyl-proline-prolinal (ZPP): Dual complementary roles for neutrophil inhibition.

Biochemical and biophysical research communications·2019
Same author

Narrative approach in understanding the drivers for resilience of military combat medics.

Journal of the Royal Army Medical Corps·2017
Same author

Mitochondria-focused gene expression profile reveals common pathways and CPT1B dysregulation in both rodent stress model and human subjects with PTSD.

Translational psychiatry·2015
Same author

Comparative analysis of phytochrome-mediated growth responses in internodes of dwarf and tall pea plants.

Planta·2014

関連する実験動画

Updated: Jul 8, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

ヘミメチル化は,E. coli のDNA複製を防ぐ.

D W Russell, N D Zinder

    Cell
    |September 25, 1987
    PubMed
    まとめ
    この要約は機械生成です。

    E. coli のDNAアデニンメチラゼ (DAM) 欠乏は,プラズミド変換を阻害する. 非メチル化プラズミッドはダムバクテリアを効率的に変換するが,半メチル化プラズミッドはそうではなく,ダムメチル化が複製の開始に不可欠であることを示している.

    さらに関連する動画

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
    09:42

    Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

    Published on: September 7, 2017

    関連する実験動画

    Last Updated: Jul 8, 2026

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
    11:19

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

    Published on: August 21, 2016

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
    09:42

    Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

    Published on: September 7, 2017

    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは
    • 微生物学 微生物学とは

    背景:

    • E. coli のDNAアデニンメチラゼ (dam) は,GATC 配列におけるアデニンをメチラする.
    • メチル化状態は,DNA複製と遺伝子発現の調節に不可欠です.
    • ダムメチラゼが不足しているE. coli菌株は,プラズミド変換が低下しています.

    研究 の 目的:

    • プラズミド変換効率におけるダムメチル化の役割を調査する.
    • ヘミメチル化および非メチル化プラズミドがダム-E. coliの変異に与える影響を決定する.
    • ダムメチレーションがDNA複製の開始に影響を与えるメカニズムを解明する.

    主な方法:

    • メチル化,ヘミメチル化,非メチル化プラズミッドを用いた変換アッセイは,ダム-E. coli.
    • 母株におけるプラズミド複製と分離の分析.
    • 変換後のプラズミドメチル化状態の特徴.

    主要な成果:

    • ヘミメチル化プラズミッドはダム・E・コライを劣等に変換し,非メチル化プラズミッドは高周波で変換する.
    • 完全にメチル化されたプラズミッドは,ダム菌株にヘミメチル化された子分子の蓄積につながります.
    • ダム+バクテリアから浄化されたプラズミッドは,特定の部位でヘミメチル化されることが多い.

    結論:

    • DNAアデニンメチラゼの活動は,E. coliにおける効率的なプラズミド変換に不可欠である.
    • ヘミメチル化は,ダム菌株におけるDNA複製開始への障壁として作用する.
    • DNAのメチル化状態,特にヘミメチル化状態は,野生型E. coliにおけるDNA複製の再始動を調節する.