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Updated: Dec 4, 2025

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

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共有した単細胞のRNAとクロマチンのプロファイリングによって識別されたクロマチンの潜在能力

Sai Ma1, Bing Zhang2, Lindsay M LaFave3

  • 1Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Biology and Koch Institute, Massachusetts Institute of Technology, Cambridge, MA 02142, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.

Cell
|October 25, 2020
PubMed
まとめ

SHARE-seqという 単細胞でのクロマチンのアクセシビリティと 遺伝子発現を測定する方法を開発しました この技術は,クロマチンの変化が細胞の運命決定に先行し,細胞の分化を理解するのに役立ちます.

キーワード:
エピゲノミクス遺伝子調節シングルセル皮膚について幹細胞

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CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

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Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

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関連する概念動画

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Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Chromatin Modification in iPS Cells

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Chromatin Position Affects Gene Expression

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The 3-dimensional positioning of chromatin in the nucleus influences the...
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Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
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Chromatin Structure and RNA Splicing

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