"Ser-Thrキナーゼにおけるアロステル活性化の古代起源"に関するコメント
Yeonwoo Park1, Jaeda E J Patton1, Georg K A Hochberg2
1Committee on Genetics, Genomics and Systems Biology, University of Chicago, Chicago, IL, USA.
Science (New York, N.Y.)
|November 20, 2020
まとめ
オーロラキナーゼは,歴史的な配列置換を通じてアロステリック調節を進化させた. しかし,この研究は,以前の発見が欠陥のある進化樹と限られたデータに基づいていたことを明らかにし,いくつかの真菌ではアロステリーが失われ,得られなかったことを示唆しています.
科学分野:
- 進化生物学
- 生物化学
- ゲノミクス
背景:
- オーロラキナーゼは 細胞分裂の重要な調節因子です
- 以前の研究によると,オーロラキナーゼは幼生生物の進化の初期にアロステル調節を進化させた.
- 祖先の配列の再構築は,祖先のタンパク質配列を推論する方法である.
研究 の 目的:
- オーロラキナーゼにおけるアロステリック調節の進化史を再評価する.
- 過去の祖先の配列再構築方法の不正確さを特定する.
- 進化の過程でアロステリック調節が得られたか失われたかを判断する.
主な方法:
- 進化樹を更新した 系統遺伝分析
- 拡張配列サンプリングによる比較ゲノミクス
- 祖先の配列を再構築する
主要な成果:
- Hadzipasic et al が使用した系統系. 信じがたいことが判明しました
- 稀少なシーケンスサンプリングは 不正確な祖先の再構築につながった.
- 再分析では,アロステリック調節は菌類のサブグループで失われ,真核生物では得られていないことが示された.
結論:
- オーロラキナーゼにおけるアロステリック調節の進化は,再評価を必要とする.
- 不正確な系統遺伝学とサンプリング方法は,誤った進化的推論につながる可能性があります.
- オーロラキナーゼにおけるアロステリック調節は,初期の真核生物の獲得ではなく,特定の真菌系統の損失を表す可能性があります.
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