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関連する概念動画

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.1K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

1.2K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
1.2K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

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Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
3.5K
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

1.8K
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
1.8K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

15.4K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
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Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

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Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
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関連する実験動画

Updated: Nov 28, 2025

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
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Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice

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多数の受容体をトランスクリプションでリンクすることで設計された正確なT細胞認識プログラム

Jasper Z Williams1,2,3, Greg M Allen1,2,3,4, Devan Shah1,2,3

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.

Science (New York, N.Y.)
|November 27, 2020
PubMed
まとめ

エンジニアリングされたT細胞は,合成のノッチ受容体を使って複数の抗原信号を統合することで,標的細胞を正確に認識します. この多受容体系は 選択的に腫瘍を標的にし 優れた細胞認識能力を発揮します

さらに関連する動画

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
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Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
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関連する実験動画

Last Updated: Nov 28, 2025

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
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Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
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Published on: October 25, 2016

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Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
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Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

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科学分野:

  • 合成生物学
  • セルラーエンジニアリング
  • 免疫学

背景:

  • 細胞認識は複数の受容体からの信号を 統合することで精度が向上します
  • 個々の分子相互作用には 複雑な生物学的認識の作業に必要な精度が 欠けていることが多い.
  • 合成生物学では 新しい細胞機能を設計するツールが提供されます

研究 の 目的:

  • 合成のノッチ受容体を使って 多受容体細胞認識回路を設計する
  • 合成T細胞が複数の抗原認識イベントを統合できるように
  • 調節可能なロジックで 精密で頑丈なセルラー認識を実現します

主な方法:

  • 合成ノッチ受容体のライブラリを設計し,トランスクリプションの相互接続を作成しました.
  • 統合された細胞外および細胞内抗原認識経路
  • 陽性と負の論理ゲートを導入した.
  • 3つの抗原とゲート回路をテストした

主要な成果:

  • 3つの異なる抗原を統合できる 合成回路を開発しました
  • 細胞の異質性に対する強度を示した.
  • エンジニアリングされたT細胞は 統合された抗原信号に基づいて 精密な認識を示しました
  • 3つの抗原とゲートは選択的に3つの抗原の腫瘍を in vivoで除去し,2つの抗原の腫瘍を保存した.

結論:

  • 合成ノッチ受容体ベースの回路は,複雑な,多受容体媒介の細胞間認識を可能にします.
  • 細胞の行動を設計する強力な戦略です 細胞の行動を設計する強力な戦略です
  • このアプローチは,特に癌の治療のために,エンジニアリングされた細胞療法における特異性と精度を高める.