関連する実験動画

Updated: Nov 25, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.2K

人体トランスクリプションエフェクターの高通量発見と特徴付け

Josh Tycko1, Nicole DelRosso2, Gaelen T Hess1

  • 1Department of Genetics, Stanford University, Stanford, CA 94305, USA.

Cell
|December 16, 2020
PubMed
まとめ

この研究では,トランスクリプションエフェクタ機能を持つ核タンパク質を特定するための新しい測定法であるHT-recruitを導入します. 新しい活性化剤と抑制剤を明らかにし 600のヒトのタンパク質を 特定した機能を備えた資源を生み出します

キーワード:
CRISPRiについてホックスクラブクロマチンの調節深層変異スキャン不明な関数の領域高通量スクリーニング哺乳類の合成生物学タンパク質領域トランスクリプションエフェクター

さらに関連する動画

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

8.3K
Massively Parallel Reporter Assays in Cultured Mammalian Cells11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

22.1K

関連する概念動画

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
81.1K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.0K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.3K