3'-尾のポケットによるRNA安定化および他のポリARNA相互作用方式

Seyed-Fakhreddin Torabi^1,2, Anand T Vaidya^1,2,3, Kazimierz T Tycowski^1,2

¹Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06536, USA.

Science (New York, N.Y.)

|January 8, 2021

まとめ

核発現のための元素 (ENEs) は,トリプルックス構造を通してRNAポリ (A) 尾を安定させる. 結晶構造は新しい相互作用を明らかにし,3'端のRNA保護機構の理解を広げている.

科学分野:

分子生物学
構造生物学
生物化学

背景:

ポリアデニレーションは,細胞のRNA機能を調節する重要なRNA変異です.
核発現のための元素 (ENEs) は,RNAトリプレックス形成を通じてポリ (A) テイルを保護する.

研究の目的:

ENE-ポリ (A) 相互作用の構造的基礎を解明する.
ENEsがポリ・ア・テイルを安定させ,RNAを保護する方法を理解する.

主な方法:

二重のENE-ポリ (A) 複合体のX線結晶学
高解像度構造分析 (2.89 アングストーム)

主要な成果:

ポリ (A) ₂₈を持つ米のHATトランポゾンENEの詳細な結晶構造.
複数のポリ (A) 相互作用モードの識別:メジャー・グルーブ・トリプルヘリックス,マイナー・グルーブ・インタラクション,およびユニークな5倍ベースモチーフ.
3端のポケットを発見した

結論:

発見は,長距離RNA相互作用に関与する既知のモチーフを拡張します.
RNA 3'端のポリアデニレーションの保護機構に関する構造的な洞察を提供します.

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