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関連する概念動画

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.4K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.4K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.1K
Homologous Recombination02:31

Homologous Recombination

58.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.5K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

4.3K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.3K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

39.5K
Overview
39.5K
Base Excision Repair01:54

Base Excision Repair

25.0K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
25.0K
このページは機械翻訳されています。他のページは英語で表示される場合があります。View in English
  1. ホーム
  3. 研究分野
  5. 生物医学と臨床科学
  7. 腫瘍学とがん発生
  9. 血液学的腫瘍
  11. Brca1とrnai因子は,小rnaとpalb2-rad52による修復を促進する.
  1. ホーム
  3. 研究分野
  5. 生物医学と臨床科学
  7. 腫瘍学とがん発生
  9. 血液学的腫瘍
  11. Brca1とrnai因子は,小rnaとpalb2-rad52による修復を促進する.

関連する実験動画

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.3K

BRCA1とRNAi因子は,小RNAとPALB2-RAD52による修復を促進する.

Elodie Hatchi1,2,3, Liana Goehring4,5,6, Serena Landini4,5,6

  • 1Department of Genetics, Harvard Medical School, Boston, MA, USA. elodie_hatchi@dfci.harvard.edu.

Nature
|February 4, 2021

PubMed で要約を見る

まとめ
この要約は機械生成です。

BRCA1-RNAi複合体によって生成される小型RNA (sdRNAs) は,Rループ部位でのDNA修復を促進する. この新しい sdRNA 修復メカニズムは静止状態と増殖状態の細胞で作用し,BRCA1媒介による腫瘍抑制に潜在的に貢献します.

さらに関連する動画

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.6K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

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関連する実験動画

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.3K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.6K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.4K

科学分野:

  • 分子生物学
  • 遺伝学
  • 生物化学

背景:

  • Rループ (RNA:DNAハイブリッド) は ゲノム不安定と人間の病気に関連しています
  • Rループは生理学的プロセスを調節し,転写終了とDNA破裂に関連しています.
  • BRCA1は単一鎖DNAの断裂から転写停止部位を保護する.

研究 の 目的:

  • R-ループ関連パウズサイトにおけるDNA修復におけるRNAの役割を調査する.
  • DNA修復に関与する小さなRNAの源と機能を特定する.
  • sdRNA媒介によるDNA修復のメカニズムを解明する.

主な方法:

  • RNA干渉 (RNAi) 経路の分析
  • 小型DNA損傷関連RNA (sdRNA) の識別と特徴付け
  • BRCA1,PALB2,RAD52を含むDNA修復に関与するタンパク質複合体の調査.

主要な成果:

  • BRCA1-RNAiタンパク質複合体は sdRNAsを生成する.
  • sdRNAは,単一鎖DNAの断裂を持つRループを含む休止部位でPALB2-RAD52複合体によるDNA修復を促進する.
  • 静止 (G0) 細胞と増殖細胞の両方で sdRNA 修復機能があります.

結論:

  • sdRNAは,ゲノム整合性の維持に関与する新種の分子を表しています.
  • sdRNA媒介によるRループ部位でのDNA修復は,ゲノム不安定を防ぐための重要な経路である.
  • このメカニズムはBRCA1の腫瘍抑制機能に寄与し,無傷の組織と幹細胞に影響を及ぼします.