関連する実験動画

Updated: Nov 17, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.5K

再プログラムされた特異性を持つボツリヌム神経毒タンパク質のファージ支援進化

Travis R Blum1,2,3, Hao Liu4,5, Michael S Packer1,2,3

  • 1Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|February 19, 2021
PubMed
まとめ

科学者はファージによる進化システムを用いて 新しい機能を持つカスタムプロテアスを設計した. この突破は特定のタンパク質を 精密に標的にし バイオテクノロジーや治療の応用を 進めてくれます

さらに関連する動画

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

264
Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation05:08

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

1.9K

関連する概念動画

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.4K
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
412
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
74.6K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
65.3K