古代植物ホメオボックス遺伝子の保存されたプレオトロピーは,シス調節解剖によって発見された
Anat Hendelman1, Sophia Zebell2, Daniel Rodriguez-Leal1
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Cell
|March 5, 2021
まとめ
トマトのWUSCHEL HOMEOBOX9 (WOX9) 遺伝子の機能を調べると,発達における隠された役割が明らかになった. 標的型変異により 深く保存されている プレオトロピク機能が発見され 農作物の改良に関する 洞察が提供されました
科学分野:
- 植物発達の遺伝学
- 進化生物学
- 分子遺伝学
背景:
- 遺伝子の機能の分散は進化の鍵ですが, 進化の深い機能的保存を評価することは困難です.
- 種間研究における限られたアレルは,保存されたプレオトロピック遺伝子の役割を覆す可能性があります.
研究 の 目的:
- 種間の WUSCHEL HOMEOBOX9 (WOX9) の機能的多様性を調査する.
- トマトの包括的なアレル系を用いて,隠されたWOX9のプレオトロプ的役割を明らかにする.
主な方法:
- トマトの総合的なアレル系を生成するために,シス調節編集駆動システムを利用した.
- アクセシブルなクロマチンと保存されたシス調節配列を分析して機能領域を特定した.
- アラビドプシスのトマトアレルを模倣し,グラウンドチェリーの親戚を調べることで保存された機能をテストしました.
主要な成果:
- トマトの発達におけるWOX9の未知のプレオトロプ的役割が明らかになった.
- 特定されたシス調節領域は,WOX9のプレオトロプ的活性に責任がある.
- グラウンドチェリーとアラビドプシスの新しい花束のフェノタイプでWOX9の保存された機能が実証された.
結論:
- 標的型のシス調節性突然変異は 深く保存された遺伝子の機能を明らかにします
- 保存されたプレオトロピーの理解は,進化的研究と作物改良の両方にとって極めて重要です.
- WOX9は,提案された種特有の役割を超えて,深く保存されたプレオトロプ的機能を表しています.
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