合成デジタル記録システムによる細胞系統のイメージング
Ke-Huan K Chow1, Mark W Budde1, Alejandro A Granados1
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Science (New York, N.Y.)
|April 9, 2021
まとめ
私たちは intMEMOIRを開発しました この新しいシステムによって 血統の記録ができます この技術により,細胞系統の関係と 発達中の生物のクローン史を再構築できます.
科学分野:
- 発達生物学
- 遺伝学
- バイオエンジニアリング
背景:
- 細胞の運命の決定は 空間的位置と系統の歴史によって 影響を受けます
- 多細胞発達の理解には 精密な系統追跡が不可欠です
研究 の 目的:
- 遺族を記録するための 新しい遺伝子システムを設計する
- 生きた細胞や組織で 血統関係やクローン史を再構築できるようにする.
主な方法:
- インテグラーゼベースのシステムであるインテグラーゼエディタブルメモリ (intMEMOIR) を光学 in situ 読み取りによる技術的変異により開発した.
- ストキャスティックと不可逆的な再結合のための独立した3つの状態の遺伝的記憶要素の配列を使用しました.
- intMEMOIRをマウスの培養細胞とドロソフィラの脳部位に適用し,血統を再構築した.
主要な成果:
- intMEMOIRは,幹細胞の系統樹を成功裏に再構築しました.
- このシステムは,59,049の異なるデジタル状態を許可し,系統情報を現地で記録しました.
- ドロソフィラの脳部位における単細胞のクローン経歴,空間的位置,および遺伝子発現の同時分析を可能にしました.
結論:
- intMEMOIRは,顕微鏡で読める系統記録のための強力なツールを提供します.
- この技術は様々な生物学的システムにおける 系統関係を分析するための基盤を確立しています
- 多細胞発達における細胞運命をより深く理解する.
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