ERからGolgiまでのタンパク質の配送は,ERから延伸する織り交わされた管状のネットワークを通して行われます

Aubrey V Weigel¹, Chi-Lun Chang¹, Gleb Shtengel¹

¹Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

Cell

|April 14, 2021

まとめ

エンドプラズマ網 (ER) は,タンパク質をゴルジ装置に輸出するための管状のネットワークを形成します. このER出口部位は,COPIIとCOPIタンパク質を通してタンパク質輸送を動的に制御します.

科学分野:

細胞生物学
分子生物学
バイオ物理学

背景:

精密なタンパク質の輸送は細胞の機能に不可欠です
エンドプラズマ網膜 (ER) からゴルギ装置へのタンパク質輸送のメカニズムは完全に理解されていません.
初期の分泌経路輸送小胞の物理的な性質は不明である.

研究の目的:

哺乳類の細胞の初期の分泌区画のダイナミックな3D構造を視覚化する.
精密にタンパク質を特定するために
輸送船の物理的性質を明らかにする.

主な方法:

全細胞,焦点イオンビームスキャニング電子顕微鏡 (FIB-SEM).
クリオ構造化照明顕微鏡 (クリオ-SIM)
ライブ・セル・シンクロナイズド・カーゴ・リリース・テクニック

主要な成果:

ERはタンパク質の輸出のための複雑で織り交わされた管状のネットワーク (ER出口部) を生成します.
この管状のネットワークは ERに隣接し薄い首で繋がっています
COPIはゴルギへの輸送を仲介する一方,COPIIは首に局所し,ERの貨物入りを規制する.

結論:

初期の分泌経路は,タンパク質の輸出のためにERから伸びるダイナミックな管状のネットワークを使用します.
このER出口サイトは,COPIIとCOPIを含む規制された構造です.
この輸送メカニズムを理解することで細胞の多様性とタンパク質の密輸に関する洞察が得られます

キーワード:

コピー COPII についてコレステロール相関光と電子顕微鏡エンドプラズマ網膜の出口部位エンドプラズマの網膜からゴルジの輸送中介体へ焦点イオンビームスキャニング電子顕微鏡膜の密輸選択フックシステムによる保持分泌経路

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