関連する実験動画

Updated: Nov 7, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Published on: September 15, 2021

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スプライスサイト m6 メチル化により,U2AF35の結合が阻害される

Mateusz Mendel1, Kamila Delaney1, Radha Raman Pandey1

  • 1Department of Molecular Biology, Science III, University of Geneva, 30 Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland.

Cell
|April 30, 2021
PubMed
まとめ

METT-10によるN6-メチラデノシン (m6A) RNA改変は,C. elegansにおけるS-アデノシルメチオニン合成酵素の前mRNAスプライシングを阻害する. この食事によって引き起こされるm6Aスイッチは,SAMの恒常性を調節し,スプライシング抑制は哺乳類で維持されます.

キーワード:
3' スプライスサイトMETT-10 についてMETTL16 についてSAM ホメオスタシスサムシンテゼU2AF35/65 についてU6 snRNA についてm ((6) A について精子生成スプライシング

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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