脂肪酸合成酵素I型によって媒介されるリン酸膜の化学酵素生成

Satyam Khanal¹, Roberto J Brea¹, Michael D Burkart¹

¹Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.

Journal of the American Chemical Society

|May 12, 2021

まとめ

研究者は新しい化学酵素法を使って基本的な構成要素から人工の脂質膜を作りましたこの突破は,アセチル-CoAとマロニル-CoAから膜に結合した小胞の de novo 形成を可能にします.

科学分野:

合成生物学
生物化学
材料科学

背景:

脂質膜の形成は合成細胞にとって不可欠ですが, in vitro で複製することは困難です.
自然のフォスフォリピド合成には復元が難しい複雑な酵素経路が含まれています.
現在の方法は単純な代謝先駆体から膜を作るのに苦労しています

研究の目的:

新しい脂質膜の生成のための化学酵素戦略を開発する.
アセチル-CoAとマロニル-CoAからフォスフォリピド膜合成を再構成する.
セルフ・アセンブリング・ベシクルのバイオミテック・ルートを作る

主な方法:

溶性細菌脂肪酸合成酵素 (cgFAS I) を利用して,パルミトイル-コアを*in situ*合成した.
脂肪酸誘導体とリソホスフォリピドの間の反応のためにネイティブ化学結合 (NCL) を採用した.
非正規のアミドフォスポリピドの自己組織化

主要な成果:

アセチル-CoAとマロニル-CoAからパルミトイール-CoAを合成した.
NCLで生成された非正規のアミドフォスポリピド
マイクロンサイズの膜に結合した膀に自己組織化が観察された.

結論:

アセチル-CoAとマロニル-CoAから直接フォスフォリピド膜の形成を初めて証明した.
酵素合成とバイオコンジュガーションを組み合わせて効率的な膀形成を行う.
合成細胞膜の生成のためのバイオミメティックアプローチを確立しました.

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