ZAR1レジストソームは,カルシウムに浸透するチャネルで,植物の免疫信号を誘発する

Guozhi Bi¹, Min Su², Nan Li²

¹State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Cell

|May 13, 2021

まとめ

植物免疫受容体ZAR1は,プラズマ膜にペンタメリック複合体,レジストソームを形成する. このレジストソームは,カルシウムに浸透するイオンチャネルとして機能し,植物の免疫反応と細胞死を引き起こします.

科学分野:

植物免疫
植物防御の分子機構
イオンチャネル機能

背景:

ヌクレオチド結合性,ルシンの豊富なリピート受容体 (NLR) は,植物や動物の重要な免疫センサーです.
アラビドプシスのNLRタンパク質ZAR1はレジストソームを形成し,植物免疫反応と細胞死を引き起こします.

研究の目的:

活性化されたZAR1レジストソームの in vivo構造と機能を調査する.
植物免疫におけるイオンチャネルとしてのZAR1レジストソームの役割を決定する.

主な方法:

単一分子画像は,ZAR1複合体をプラズマ膜で視覚化します.
クセノプスの卵細胞と平面の脂質二層の電気生理学的記録
ZAR1媒介によるCa2+の流入,活性酸素種の生成,および植物細胞の細胞死亡の分析.

主要な成果:

活性化されたZAR1は,プラズマ膜にペンタメリック複合体を形成する.
ZAR1レジストソームは,Glu11残留に依存する,カルシウム浸透性カチオンチャネル活性を示す.
植物におけるZAR1の活性化は,Ca2+の流入,ROSの産生,細胞死につながる.

結論:

ZAR1レジストソームは,カルシウムに浸透するカチオンチャネルとして機能する.
このチャネル活動は,ZAR1媒介による植物免疫と細胞死誘導に極めて重要です.

キーワード:

アラビドプシス NLR についてシュードモナスクサントモナスカルシウムチャンネル細胞死病気に対する耐性植物免疫レジストソーム単一分子イメージング

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