アイソコートクスとヒポカンプス形成のトランスクリプトーム細胞の分類

Zizhen Yao¹, Cindy T J van Velthoven¹, Thuc Nghi Nguyen¹

¹Allen Institute for Brain Science, Seattle, WA 98109, USA.

Cell

|May 18, 2021

まとめ

哺乳類の脳の同皮質と海馬形成は,同じ細胞組織を共有し,同種のニューロンタイプを明らかにする. この研究は脳の発達と機能の理解に影響を及ぼす分子構造をマッピングします

科学分野:

神経科学
細胞生物学
ゲノミクス

背景:

イソコーテックスとヒポカンプス形成 (HPF) は,知覚,認知,感情,学習などの哺乳類の脳機能に不可欠です.
これらの脳の領域の細胞の多様性と組織を理解することは神経科学にとって根本的なことです

研究の目的:

成人マウスの同皮質とHPFの包括的なトランスクリプトミックの細胞型分類を作成する.
同皮質とHPFの間の細胞組織と進化的関係を調査する.
これらの脳の構造における細胞の種類の大規模な変化を特定するためです

主な方法:

成人マウスの同皮質とHPFから約130万個の細胞の単細胞RNA配列解析.
生物情報分析により,細胞型の複写分類を導き出します.
同皮質とHPFの間の細胞型レパートリーの比較分析.

主要な成果:

同皮質とHPFにおけるグルタマタージックおよびGABAergicニューロンタイプの包括的なカタログが作成されました.
以前の見解とは対照的に,HPFは6層の同皮質に同類するグルタマタージックニューロンタイプを有することが判明した.
皮質の深さに沿って,皮質のシートにわたって,そして海馬とサブキュラムの内部で,細胞の連続した変異が特定されました.

結論:

アイソコーテックスとHPFは,HPFのシンプルさに関する伝統的な見解に異議を唱える共通の基盤の回路組織を共有しています.
この研究は,哺乳類の同皮質とHPFの分子構造を提供する.
これらの重要な脳の構造の発達,進化,接続性,機能についての洞察を提供しています

キーワード:

GABAアレルギー性細胞タイプ皮質興奮神経グルタマタージックヒポカンプスインターニューロン単細胞RNAシーケンシング単細胞トランスクリプトミクス

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