RNAリガース活性を持つトリエース核酸酵素

Yao Wang¹, Yueyao Wang^1,2, Dongfan Song³

¹State Key Laboratory of Coordination Chemistry, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, Jiangsu 210023, China.

Journal of the American Chemical Society

|May 24, 2021

まとめ

トレオース核酸 (TNA) 酵素は,潜在的なRNA前駆体としての役割を調査するために分離されました. これらのTNA酵素は,特定のRNA結合を触媒化し,TNAをサポートします.

科学分野:

生命研究の起源
合成生物学
生物化学

背景:

トレオース核酸 (TNA) は,RNAのより単純な代替核酸である.
TNAは塩基配列の性質を示し,生命の初期にRNAに先行した可能性がある.
触媒性TNA配列は RNAの世界への移行を容易にしたかもしれません

研究の目的:

RNAリガース活性を持つTNA酵素を分離し,特徴づけること.
RNA前遺伝子ポリマーとしてのTNAの可能性を探求するためです
バイオテクノロジーのためのTNAベースの分子ツールを開発する.

主な方法:

in vitro選択を用いたRNAリガゼTNA酵素の分離
TNA酵素T8-6の触媒活性の特徴
TNA酵素基板の特異性と結合効率の評価

主要な成果:

TNA酵素T8-6が分離され,RNAリガース活性を示した.
T8-6は1.1 × 10^-2 min^-1のコブスを持つ2'-5'フォスフォースター結合の形成を触媒とする.
T8-6は,UA矢の結合を必要とするRNA基板を効率的に結合し,ハンマーヘッドリボ酵素のような機能的なRNAの合成を可能にします.

結論:

実験的な証拠は,TNAが合理的な RNA前遺伝子ポリマーであることを支持しています.
TNA酵素は生物技術の応用のための新しい分子ツールを提供します.
この研究はTNAからRNAの世界への移行の洞察を提供します.

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