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In Vitro Model of Coronary Angiogenesis
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静脈内皮細胞の発達および病理的血管新生における役割
Heon-Woo Lee1, Yanying Xu1,2, Liqun He3
1Yale Cardiovascular Research Center (H.-W.L., Y.X., S.-W.J., M.S.), Yale University School of Medicine, New Haven, CT.
Circulation
|September 3, 2021
まとめ
静脈内皮細胞は正常な発達と疾患の両方で新しい血管の成長の主な原動力です. これらの細胞は 血流に反して移動し 新しい血管ネットワークを形成し 血管新生における 重要な役割を強調しています
科学分野:
- 血管生物学
- 内皮細胞生物学
- 発達生物学
背景:
- 血管ネットワークの拡大である血管新生は 生理学的および病理学的条件において極めて重要です
- 血管新生を開始する特定の内皮細胞サブタイプは,まだよくわかっていません.
- 新しい血管形成に毛細血管,静脈,動脈内皮細胞の貢献を区別することは不可欠です.
研究 の 目的:
- 血管新生を促す 主要な内皮細胞サブタイプを特定する
- 血管の正常な発達と病的な発達の両方で異なる内皮細胞サブタイプの役割を調査する.
- 血管ネットワークの拡大中の内皮細胞の起源と行動を解明する.
主な方法:
- 内皮細胞のサブタイプ特異的なマーカーを用いた遺伝的運命マッピングを用いた.
- 静脈内皮特異的遺伝子発現のための新しい誘導性マウスラインを開発した (Gm5127を使用).
- イントラバイタル2フォトン画像を用いて,内皮細胞の移動を in vivo で観察した.
主要な成果:
- 静脈内皮細胞は,血管新生性血管拡張の主要なサブタイプとして特定されました.
- 静脈内皮細胞が増殖し,血流に反して移動し,他の内皮細胞サブタイプに微分化することが観察された.
- 静脈内皮細胞の病理的血管新生における重要な役割を示した. 静脈内皮細胞の異常形成と酸素誘発の網膜病変を含む.
結論:
- 静脈内皮細胞は生理学的および病理学的血管新生の両方の主要な原動力です.
- これらの発見は,血管の発達と疾患における静脈内皮の中心的な重要性を強調しています.
- この研究は,血管新生過程で新たに形成された血管系の細胞起源を明らかにします.
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